P0270

Calmodulin from bovine heart

lyophilized powder, 2,500-10,000 units/mg protein

• 동의어(들): Phosphodiesterase 3′:5′-cyclic nucleotide activator from bovine heart
• CAS Number: 77107-46-1
• MDL number: MFCD00131869
• UNSPSC 코드: 12352202
• NACRES: NA.61

속성

• 생물학적 소스: bovine heart
• Quality Level: 200
• 형태: lyophilized powder
• 특이 활성도: 2,500-10,000 units/mg protein
• 구성: Protein, ≥85% Lowry
• 기술: calcium flux assay: suitable
• UniProt 수납 번호: P62157
• 저장 온도: −20°C
• 유전자 정보: cow ... CALM3(520277)

설명

애플리케이션

This is the protein activator required for the Ca²⁺ dependent activation of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520.

생화학적/생리학적 작용

Ca²⁺ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca²⁺ homeostasis.

단위 정의

One unit will stimulate 0.008 activated unit of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520, in a 3 mL reaction volume at pH 7.5 and 30 °C, to 50% of the maximum activity of the enzyme when saturated with activator, in the presence of 0.01 mM Ca²⁺.

물리적 형태

Lyophilized powder containing buffer salts as imidazole and magnesium sulfate

제조 메모

Purified by a modification of the procedure of Teo, T.S., et al., J. Biol. Chem., 248, 588 (1973).

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)