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Merck
모든 사진(1)

주요 문서

P4439

Sigma-Aldrich

Phosphatase, Alkaline from porcine kidney

lyophilized powder, ≥100 DEA units/mg protein

동의어(들):

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
eCl@ss:
42010105
NACRES:
NA.54

가격 및 재고 정보를 현재 이용할 수 없음

양식

lyophilized powder

특이 활성도

≥100 DEA units/mg protein

구성

Protein, ≥50%

저장 온도

−20°C

애플리케이션

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling[1].

생화학적/생리학적 작용

Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.

포장

Package size based on DEA units

단위 정의

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

물리적 형태

Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0

분석 메모

Protein determined by biuret.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Sequencing end-labeled DNA with base-specific chemical cleavages.
A M Maxam et al.
Methods in enzymology, 65(1), 499-560 (1980-01-01)
Naomi Ofer et al.
The Journal of biological chemistry, 288(16), 11287-11293 (2013-03-09)
Protein degradation via prokaryotic ubiquitin-like protein (Pup) tagging is conserved in bacteria belonging to the phyla Actinobacteria and Nitrospira. The physiological role of this novel proteolytic pathway is not yet clear, although in Mycobacterium tuberculosis, the world's most threatening bacterial
Insulin affects dopamine metabolism in Drosophila females under normal and stress conditions.
N V Adonyeva et al.
Doklady. Biochemistry and biophysics, 448, 40-42 (2013-03-13)
Lars Hansen et al.
American journal of human genetics, 92(4), 575-583 (2013-04-09)
PGAP2 encodes a protein involved in remodeling the glycosylphosphatidylinositol (GPI) anchor in the Golgi apparatus. After synthesis in the endoplasmic reticulum (ER), GPI anchors are transferred to the proteins and are remodeled while transported through the Golgi to the cell
Gouri Chaudhuri et al.
Indian journal of biochemistry & biophysics, 50(1), 64-71 (2013-04-27)
The hydrolysis of p-nitrophenyl phosphate (pNPP) by calf intestinal alkaline phosphatase (CIAP) was investigated with respect to kinetic parameters such as V(max), K(m) and K(cat) under varying pH, buffers, substrate concentration, temperature and period of incubation. Highest activity was obtained

프로토콜

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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