P6746

Anti-PML antibody, Mouse monoclonal

clone PML-97, purified from hybridoma cell culture

• 동의어(들): Anti-Promyelocytic Leukemia
• MDL number: MFCD06412254
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: PML-97, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~90 kDa
• 종 반응성: hamster, human, mouse
• 기술: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using 293T cells expressing recombinant PML3
• 동형: IgG1
• UniProt 수납 번호: P29590
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PML(5371)

설명

일반 설명

Monoclonal Anti-PML (mouse IgG1 isotype) is derived from the PML-97 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with recombinant full-length human PML (promyelocytic leukemia) protein. PML is characterized with cysteine-rich zinc-binding domains (RING), two B-boxes, an adjacent leucine coiled coil forming the RBCC or tripartite motif (TRIM), and a nuclear localization signal (NLS). The RING and B-box are important for the interaction of the PML protein with different proteins. In normal cells, the PML protein is found in nuclear bodies (PML NBs) whereas in acute promyelocytic leukemia (APL) patients, the PML NBs are disrupted and delocalized from PML NBs to nucleus and cytoplasm.

The PML gene is located on the human chromosome at 15q24.1.

특이성

Monoclonal Anti-PML recognizes human, mouse, and hamster PML (about 90 kDa)

면역원

recombinant full-length human PML (promyelocytic leukemia) protein.

애플리케이션

Anti-PML antibody, Mouse monoclonal may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunocytochemistry
• indirect immunofluorescence

생화학적/생리학적 작용

A monoclonal antibody to promyelocytic leukemia (PML) is an important tool for studying PML function in normal and cancerous cells. In normal cells, the PML protein and the PML nuclear bodies play a role in different physiological processes such as: growth control, transcription, DNA repair, DNA replication and RNA transport.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)