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Merck
모든 사진(2)

주요 문서

P6912

Sigma-Aldrich

o-Phenylenediamine dihydrochloride

peroxidase substrate, chromogenic, tablet

동의어(들):

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
Molecular Weight:
181.06
Beilstein:
3912045
MDL number:
UNSPSC 코드:
12352204
PubChem Substance ID:
NACRES:
NA.83

제품명

o-Phenylenediamine dihydrochloride, tablet, 5 mg substrate per tablet

Quality Level

양식

tablet

mp

258 °C (dec.) (lit.)

저장 온도

2-8°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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일반 설명

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

애플리케이션

o-Phenylenediamine dihydrochloride has been used as a peroxidase substrate in ELISA (enzyme-linked immunosorbent assay).

포장

150 mg total tablet weight

재구성

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

픽토그램

Health hazardExclamation markEnvironment

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서

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