P6912
o-Phenylenediamine dihydrochloride
peroxidase substrate, chromogenic, tablet
동의어(들):
1,2-Phenylenediamine tablet, OPD, OPD Tablet
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모든 사진(2)
About This Item
Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
Molecular Weight:
181.06
Beilstein:
3912045
MDL number:
UNSPSC 코드:
12352204
PubChem Substance ID:
NACRES:
NA.83
추천 제품
일반 설명
o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.
애플리케이션
o-Phenylenediamine dihydrochloride has been used as a peroxidase substrate in ELISA (enzyme-linked immunosorbent assay).
포장
150 mg total tablet weight
재구성
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
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문서
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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