P8804

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)

• 동의어(들): 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
• CAS Number: 37288-19-0
• 효소 위원회 번호: 4.6.1.13 (BRENDA, IUBMB)
• EC Number: 232-638-2
• MDL number: MFCD00131896
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 생물학적 소스: Bacillus sp. (Bacillus cereus)
• Quality Level: 200
• 양식: buffered aqueous glycerol solution
• 특이 활성도: ≥1,000 units/mg protein (Lowry)
• 분자량: 28 kDa
• 외래 활성: Phopholipase C (lecithinase) ≤1 units/mg protein; Sphingomyelinase ≤40 units/mg protein
• 저장 온도: 2-8°C
• 유전자 정보: Bacillus cereus E33L ... BCZK3513(3026815)

설명

일반 설명

Phospholipase C (PI-PLC) from Bacillus cereus has irregular triosephosphate isomerase (TIM)-barrel structure with eight-standard parallel β barrel. It is a 28 kDa protein.

애플리케이션

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:

• in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)
• to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membrane
• to cleave immunolabeled HeLa cells

생화학적/생리학적 작용

Phospholipase C (PI-PLC) releases diacylglycerol by cleaving the glycosylphosphatidylinositol. It has broad substrate specificity and its activity is influenced by metal ions and surfactants.

Used for the release of GPI anchored proteins from the membrane.

단위 정의

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

물리적 형태

Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA

분석 메모

Acetylcholinesterase is measured according to Ellman, et al.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)