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Merck
모든 사진(1)

주요 문서

PNS1010

Sigma-Aldrich

Stabilyser Reagent

for stabilization of DNA, RNA, and protein in tissue extracts

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About This Item

UNSPSC 코드:
41116012
NACRES:
NA.77

양식

liquid

Quality Level

특징

for stabilization of DNA, RNA, and protein in tissue extracts

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

The stability of protein and antibody structure and function is dependent on a variety of factors such as temperature, freeze/thaw cycles, proteases and pH. For some proteins and antibodies, function can be lost completely with a single freeze/thaw cycle and proteases can significantly degrade proteins and antibodies within hours even when kept at 4 °C. Stabilyser Reagent minimizes the risk to improve protein assay quality and accuracy.

Stabilyser Reagent is a proprietary formulation of detergents and solutes optimized for efficient extraction, stabilization and storage of protein, DNA, and RNA from tissue samples. The unique composition of Stabilyser enables isolation of functionally active proteins, while maintaining integrity of nucleic acids. Stabilization of proteins and RNA is achieved even after 24 hours at room temperature Stabilyser reagent is compatible with Western blotting protocols, functional protein activity assays, and nucleic acid applications like qPCR.

Use in conjunction with a homognenizer for simultaneous tissue lysis and stabilization of protein and nucleic acids in tissue samples.

애플리케이션

Prevents degradation of proteins and nucleic acid during tissue lysis and storage. Stabilize functional proteins and RNA.

특징 및 장점

  • Gentle, non-denaturing lysis buffer does not interfere with downstream applications
  • Convenient ready-to-use reagent
  • Stabilizes RNA and protein for analysis at a later date

제조 메모

Ready-to-use reagent. In general it is recommended to start optimization with 20 μL of Stabilyser reagent per mg of tissue.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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관련 콘텐츠

세제 용해화, 냉동-해동 용해, 삼투압 충격, 음파파쇄, 효소성 세포 용해 및 Dounce, Polytron 그리고 절구와 절구공이 균질화와 같은 기계적 파쇄법을 포함한 세포 용해와 단백질 추출법의 개요.

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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