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Merck
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주요 문서

PP0530

Sigma-Aldrich

GlycoProfile 2-AA Labeling Kit

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About This Item

EC Number:
UNSPSC 코드:
12352200
NACRES:
NA.32

일반 설명

The GlycoProfile labeling kits are designed for efficient labeling of N-linked, O-linked, and glycosyl­phosphatidyl­inositol (GPI) anchored glycans with either 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). These small fluorophores increase the spectral absorption of glycans, improving the detection of labeled glycans by HPAE (high-performance anion exchange chromatography), HPLC, and ESI-MS (electrospray mass spectroscopy) methods. Binding is robust and the resulting dye-glycan conjugates are stable, with no degradation during post-labeling analysis.

애플리케이션

GlycoProfile 2-AA Labeling Kit has been used in the labeling of fluorescently derivatized N-glycans from chinese hamster ovary CHO cells.
While 2-AB is slightly less sensitive than 2-AA, it is suitable for downstream glycan analysis by HPAE, HPLC and ESI-MS. Although suitable for the same applications, 2-AA is better suited for SDS-PAGE.

법적 정보

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

키트 구성품 전용

제품 번호
설명

  • Anthranilic acid (2-AA) 2 x 6

  • Dimethyl sulfoxide (DMSO) 2 x 350

  • Acetic acid, glacial 2 x 200

  • Reductant (sodium cyanoborohydride) 2 x 6

픽토그램

Corrosion

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

302.0 °F - closed cup

Flash Point (°C)

150 °C - closed cup


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문서 라이브러리 방문

Modulation of IgG1 immunoeffector function by glycoengineering of the GDP-fucose biosynthesis pathway
Kelly RM, et al.
Biotechnology and Bioengineering, 115(3), 705-718 (2018)
Weiguo Fang et al.
Plant physiology, 154(3), 1549-1557 (2010-09-15)
The symbiotic associations between rhizospheric fungi and plants have enormous environmental impact. Fungi are crucial to plant health as antagonists of pathogens and herbivores and facilitate the uptake of soil nutrients. However, little is known about the plant products obtained

문서

Glycoprofile Labeling Kits efficiently label glycans for glycan analysis, offering flexibility in choice of labeling.

Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.

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