추천 제품
생물학적 소스
synthetic (organic)
Quality Level
설명
RGD Peptide for Cell Adhesion, 5 mg
무균
electron beam irradiated
분석
≥95% (HPLC)
형태
powder
기술
tissue culture: suitable
배송 상태
wet ice
저장 온도
2-8°C
InChI
1S/C98H174N34O30/c1-48(2)31-60(84(151)120-56(79(100)146)22-17-27-107-96(101)102)124-86(153)62(33-50(5)6)126-88(155)64(35-52(9)10)128-89(156)65(36-53(11)12)127-87(154)63(34-51(7)8)125-85(152)61(32-49(3)4)123-83(150)59(24-19-29-109-98(105)106)122-91(158)67(44-133)119-76(142)42-113-73(139)39-111-72(138)38-112-74(140)40-114-82(149)58(21-15-16-26-99)121-92(159)69(46-135)130-93(160)68(45-134)129-80(147)54(13)116-94(161)71-25-20-30-132(71)95(162)70(47-136)131-90(157)66(37-78(144)145)118-77(143)43-115-81(148)57(23-18-28-108-97(103)104)117-75(141)41-110-55(14)137/h48-54,56-71,133-136H,15-47,99H2,1-14H3,(H2,100,146)(H,110,137)(H,111,138)(H,112,140)(H,113,139)(H,114,149)(H,115,148)(H,116,161)(H,117,141)(H,118,143)(H,119,142)(H,120,151)(H,121,159)(H,122,158)(H,123,150)(H,124,153)(H,125,152)(H,126,155)(H,127,154)(H,128,156)(H,129,147)(H,130,160)(H,131,157)(H,144,145)(H4,101,102,107)(H4,103,104,108)(H4,105,106,109)/t54-,56-,57-,58-,59-,60-,61-,62-,63-,64-,65-,66-,67-,68-,69-,70-,71-/m0/s1
InChI key
DULMZMHLQQZGPY-ACFXXXONSA-N
애플리케이션
INSTRUCTIONS FOR USE:
Use these procedures as a guideline to determine the optimal coating conditions for the culture system of coice. To maintain sterility, perform all operations in a laminar flow hood. Two options are provided:
Procedure A
1. Remove cap and add 5 ml of serum-free medium or PBS to the bottle.
2. Replace cap and vortex contents vigorously. Ensure that the PEPTITE-2000 is completely solubilized. The solution will remain slightly hazy.
3. Transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using serum-free medium or PBS. A typical working concentration may range from 0.1 to 10 μg/ml.
4. Add appropriate amount of diluted material to culture surface.
5. Incubate at room temperature or 37 °C, covered, for 1-2 hours.
6. After incubation, aspirate remaining material.
7. Rinse plates carefully with water and avoid scratching bottom surface of plates.
8. Plates are ready for use. They may also be stored at 2-8 °C damp or air dried if sterility is maintained.
9. Store remaining solubilized PEPTITE-2000 at 2 to 10 °C.
Additional note: Include divalent cations (Calcium, Magnesium, or Manganese) in cell attachment solution to obtain optimum cell binding.
Procedure B
1. Remove cap and add 5 ml of sterile 70% Ethanol
2. Replace cap and vortex contents. Ensure that the PEPTITE-2000 is completely solubilized.
3. Transfer desired volume of solution from the bottle to a dilution vessel. Dilute to the desired concentration using 70% Ethanol. Concentrations from 0.1 to 10 μg/ml should be tested.
4. Add appropriate amount of diluted material to culture surface.
5. Leave the coated container, uncovered, in a laminar flow hood until the wells are dry.
6. Rinse plates carefully with water and avoid scratching bottom surface of plates.
7. Plates are ready for use.
8. Store remaining solubilized PEPTITE-2000 at 2-10 °C.
Use these procedures as a guideline to determine the optimal coating conditions for the culture system of coice. To maintain sterility, perform all operations in a laminar flow hood. Two options are provided:
Procedure A
1. Remove cap and add 5 ml of serum-free medium or PBS to the bottle.
2. Replace cap and vortex contents vigorously. Ensure that the PEPTITE-2000 is completely solubilized. The solution will remain slightly hazy.
3. Transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using serum-free medium or PBS. A typical working concentration may range from 0.1 to 10 μg/ml.
4. Add appropriate amount of diluted material to culture surface.
5. Incubate at room temperature or 37 °C, covered, for 1-2 hours.
6. After incubation, aspirate remaining material.
7. Rinse plates carefully with water and avoid scratching bottom surface of plates.
8. Plates are ready for use. They may also be stored at 2-8 °C damp or air dried if sterility is maintained.
9. Store remaining solubilized PEPTITE-2000 at 2 to 10 °C.
Additional note: Include divalent cations (Calcium, Magnesium, or Manganese) in cell attachment solution to obtain optimum cell binding.
Procedure B
1. Remove cap and add 5 ml of sterile 70% Ethanol
2. Replace cap and vortex contents. Ensure that the PEPTITE-2000 is completely solubilized.
3. Transfer desired volume of solution from the bottle to a dilution vessel. Dilute to the desired concentration using 70% Ethanol. Concentrations from 0.1 to 10 μg/ml should be tested.
4. Add appropriate amount of diluted material to culture surface.
5. Leave the coated container, uncovered, in a laminar flow hood until the wells are dry.
6. Rinse plates carefully with water and avoid scratching bottom surface of plates.
7. Plates are ready for use.
8. Store remaining solubilized PEPTITE-2000 at 2-10 °C.
PEPTITE-2000® is suitable to coat:
- paramagnetic beads prior to incorporation with embryonic stem cells
- Corning Transwell polycarbonate membrane inserts for promoting cell attachment
- tosyl-activated magnetic beads in human colonic epithelial cells (HT-29) for binding experiments
- gold nanorods
특징 및 장점
RGD PEPTITE-2000 is used to coat tissue culture plasticware for enhanced cell attachment and adhesion. The product has been specifically developed for ease-of-use for the researcher. It is provided in a 5 mg package size to provide sufficient quantity of product for coating a large surface area. The product has been sterilized and is ready-to-use after proper dilution. The optimal concentration for cell attachment and culture may differ for various cell types. Some experimentation will be required to determine the optimal conditions for individual cell culture systems. A typical working concentration may range from 1 to 100 μg/ml.
기타 정보
The tripeptide Arg-Gly-Asp (RGD) is an important protein sequence in the binding of proteins to cell surfaces. The RGD motif was initially identified in fibronectin as the minimal sequence that mediates cell attachment. Research has also shown that the RGD motif in present in other proteins such as vitronectin, osteopontin, collagens, thrombospondin, fibrinogen and many other factors. Many adhesive cell surface receptors recognize the RGD sequence for cell attachment.
Ac-Gly-D-Arg-Gly-Asp-Ile-Pro-Ala-Ser-Ser-Lys-Gly-Gly-Gly-Gly-Ser-D-Arg-Leu-Leu-Leu-Leu-Leu-Leu-D-Arg-NH2
품질
Identity and purity qualitatively determined by SDS-PAGE
법적 정보
PEPTITE-2000 is a registered trademark of Advanced BioMatrix, Inc.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Laura R Geuss et al.
PloS one, 9(12), e113982-e113982 (2014-12-17)
Mechanical forces play an important role in proper embryologic development, and similarly such forces can directly impact pluripotency and differentiation of mouse embryonic stem cells (mESC) in vitro. In addition, manipulation of the embryoid body (EB) microenvironment, such as by
Philipp Nicol et al.
Scientific reports, 10(1), 8227-8227 (2020-05-20)
Neoatherosclerosis represents an accelerated manifestation of atherosclerosis in nascent neointima after stenting, associated with adverse events. We investigated whether improved reendothelialization using RGD-coated stents results in diminished vascular permeability and reduced foam cell formation compared to standard DES in atherosclerotic rabbits.
Masaya Saito et al.
Digestive diseases and sciences, 57(8), 2022-2030 (2012-04-03)
Genome-wide association studies have revealed a link between autophagy-related (ATG) genes and susceptibility to Crohn's disease. This suggests underlying involvement of autophagy impairment in the pathogenesis of Crohn's disease. This study was performed to investigate the pathophysiological importance of autophagy
Sandra P Sanchez-Rodriguez et al.
Biotechnology and bioengineering, 113(10), 2228-2240 (2016-08-27)
Remote activation of specific cells of a heterogeneous population can provide a useful research tool for clinical and therapeutic applications. Here, we demonstrate that photostimulation of gold nanorods (AuNRs) using a tunable near-infrared (NIR) laser at specific longitudinal surface plasmon
Fuxiang Wei et al.
Nature communications, 11(1), 4902-4902 (2020-10-01)
Living cells and tissues experience various complex modes of forces that are important in physiology and disease. However, how different force modes impact gene expression is elusive. Here we apply local forces of different modes via a magnetic bead bound
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