S8320

SigmaMarker^™

high range, mol wt 36,000-200,000 Da

• MDL number: MFCD00165844
• UNSPSC 코드: 12352200

속성

• 형태: lyophilized powder
• 사용: sufficient for 10-20 large gels (16 × 18 cm); sufficient for 20-30 mini-gels (10 × 10 cm); vial sufficient for 50-100 PhastGels
• 분자량: 36,000-200,000 Da
• 저장 온도: 2-8°C

설명

애플리케이션

SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.

기타 정보

Protein (mol. wt.)
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
Ovalbumin, chicken egg (45,000)
Glutamic dehydrogenase, bovine liver (55,000)
Albumin, bovine serum (66,000)
Phosphorylase B, rabbit muscle (97,000)
β-Galactosidase, E. coli (116,000)
Myosin, porcine heart (200,000)

Will yield bands for the following seven proteins from the SigmaMarker list with Coomassie Blue staining.

재구성

Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue.

법적 정보

SigmaMarker is a trademark of Sigma-Aldrich Co. LLC

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)