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생물학적 소스
abalone entrails
Quality Level
유형
Type VIII
양식
lyophilized powder
특이 활성도
20-40 units/mg solid
분자량
~43 kDa by gel filtration
외래 활성
β-Glucuronidase ≤10.0 Sigma units/mg solid
저장 온도
−20°C
애플리케이션
Sulfatase from abalone entrails has been used in a study to determine that human p-selectin glycoprotein ligand-1 interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus. Sulfatase from abalone entrails has also been used in a study to investigate HPLC of sulfate and glutathione conjugates from hamster embryo fibroblasts.
Sulfatase from abalone entrails has been used to digest the conjugated forms into free forms for the analysis of tissue green tea polyphenols (GTPs). It has also been used for the detection of free, glucuronidated or total form of GTPs.
생화학적/생리학적 작용
Sulfatases hydrolyze the sulfate esters of complex macromolecules, like glycosaminoglycans and sulfatides. It helps to modulate the sulfation states that determine the action of several physiological molecules.
단위 정의
One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C (30 min assay).
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Quercetin increased bioavailability and decreased methylation of green tea polyphenols in vitro and in vivo
Wang P, et al.
Food, 3(6), 635-642 (2012)
Sulfatases: structure, mechanism, biological activity, inhibition, and synthetic utility
Hanson SR, et al.
Angewandte Chemie (International ed. in English), 43(43), 5736-5763 (2004)
B A Merrick et al.
Carcinogenesis, 6(9), 1303-1307 (1985-09-01)
Chromatography of benzo[a]pyrene (BaP) sulfate, glucuronide and glutathione (GSH) conjugate standards were examined by h.p.l.c. on a C8 column as modified by various organic acids and solvents. Sulfate and glucuronide standards were positional isomers derived from BaP-1,3,6,7,9 phenols and BaP-GSH
Use of a Phage Display Antibody to Measure the Enzymatic Activity of the Sulfs
Uchimura K, et al.
Test, 480, 51-64 (2010)
Jennifer L Donovan et al.
The British journal of nutrition, 87(1), 31-37 (2002-03-16)
Little is known about flavonoid metabolism and excretion in man. In the present study, the urinary excretion of a major flavonoid in wine, catechin, and its metabolites, were measured after nine human subjects each consumed 120 ml red wine (RW)
프로토콜
Enzymatic Assay of Sulfatase (EC 3.1.6.1.)
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