SAB4200658

Monoclonal Anti-EPS8 antibody produced in mouse

clone EPS8-2, purified from hybridoma cell culture

• 동의어(들): DFNB102, epidermal growth factor receptor pathway substrate 8
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: EPS8-2
• 형태: buffered aqueous solution
• 분자량: antigen ~90 kDa
• 종 반응성: human
• 농도: ~1 mg/mL
• 기술: flow cytometry: 10-20 μg/test using using A549 cells.; immunoblotting: 2.5-5 μg/mL using using whole extracts of HepG2 cells.; immunofluorescence: 10-20 μg/mL using using whole extracts of A549 cells.; immunoprecipitation (IP): suitable
• 동형: IgG2a
• UniProt 수납 번호: Q12929
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... EPS8(2059)

설명

일반 설명

Monoclonal Anti- EPS8 (mouse IgG2a isotype) is derived from the hybridoma EPS8-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the N-terminal region of human EPS8 (GeneID: 2059), conjugated to Key loop hemocyanin (KLH). Epidermal growth factor receptor kinase substrate 8 (EPS8) is also known as DFNB102 (autosomal recessive deafness-102). EPS8 is also found to be an actin filament barbed end capping protein.

면역원

synthetic peptide corresponding to a sequence at the N-terminal region of human EPS8

애플리케이션

Monoclonal Anti-EPS8 antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence
• flow cytometry

생화학적/생리학적 작용

Epidermal growth factor receptor kinase substrate 8 (EPS8) serves as a substrate for the kinase activity of epidermal growth factor receptor (EGFR). It is essential for cell motility and regulation of actin filaments growth. EPS8 scaffolds together with Abl interactor 1 (Abi1) and SOS Ras/Rac guanine nucleotide exchange factor 1 (Sos1) to a trimeric complex which is required for activation of the small guanosine triphosphatase (GTPase) Rac. On the other hand, EPS8 form complexes with related to the N terminus of tre oncogene (RN-tre), a Rab5 GTPase activating protein, to inhibit EGFR internalization. EPS8 is involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration, cancer cell migration and invasion. It is reported to be expressed at elevated levels in a range of human malignancies. This protein is also involved in spine morphogenesis and plasticity and may characterize forms of intellectual disabilities. For instance, reduced EPS8 levels were reported in brains of autism patients.

물리적 형태

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable