추천 제품
항체 형태
purified from hybridoma cell culture
Quality Level
클론
EXO-68
양식
liquid
종 반응성
Pseudomonas aeruginosa
농도
~1 mg/mL
기술
ELISA: 2.5-5 μg/mL using Exotoxin A from Pseudomonas aeruginosa for coating.
immunoblotting: 0.125-0.25 μg/mL using exotoxin A from Pseudomonas aeruginosa.
동형
IgM
저장 온도
−20°C
타겟 번역 후 변형
unmodified
일반 설명
Pseudomonas aeruginosa is a rod shaped, gram negative, monoflagellated, aerobic to facultative anaerobe bacteria which commonly inhabits soil and aqueous environments.1,2 Pseudomonas Exotoxin A (PE) is the most potent virulence factor secreted by some strains of P. aeruginosa It is composed of three structural domains, N-terminal domain (I) responsible for the toxin binding to its host cell receptor, middle domain (II) has a role in toxin translocation across the membrane, and C-terminal domain (III) which has ADP-ribosylation activity.4,5
특이성
Monoclonal Anti-Exotoxin A from Pseudomonas aeruginosa antibody specifically recognizes Exotoxin A from Pseudomonas aeruginosa (ETA, PE) and has no cross reactivity with staphylococcal enterotoxin A and B (SEA, SEB) and cholera toxin.
애플리케이션
The antibody may be used in various immunochemical techniques including Immunoblotting (70 kDa) and ELISA.
생화학적/생리학적 작용
P.aeruginosa is considered an opportunistic human pathogen mainly causing disease in immunocompromised patients. It is especially fatal in cystic fibrosis (CF) patients, but also presents a major problem in chronic wounds, burn wounds and infection of implanted biomaterials such as catheters.3The genome of P. aeruginosa encodes a vast arsenal of virulence factors such as, Type 3 secretion system (T3SS), type 4 pilli and several secreted proteases, lipases and phospholipases.1 The Exotoxin A ADP-ribosylation activity inhibits host elongation factor 2 (EF2), and protein synthesis.1 Due to its toxin ADP-ribosylation activity PE is considered as a selective agent for the elimination of specific cell populations resulting in the irreversible shut down of protein synthesis leading to cell death. To reduce the adverse effects of natural PE, mutated PE was used in several attempts to develop recombinant toxin-antibody or cytokines fusion fragments for therapeutic application including cancer immunotherapy.5-9
물리적 형태
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
저장 및 안정성
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
면책조항
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Science (New York, N.Y.), 254(5035), 1173-1177 (1991-11-22)
Recombinant toxins target cell surface receptors and antigens on tumor cells. They kill by mechanisms different from conventional chemotherapy, so that cross resistance to conventional chemotherapeutic agents should not be a problem. Furthermore, they are not mutagens and should not
Infection and immunity, 69(11), 6962-6969 (2001-10-13)
Pseudomonas aeruginosa is the major infectious agent of concern for cystic fibrosis patients. Strategies to prevent colonization by this bacterium and/or neutralize its virulence factors are clearly needed. Here we characterize a dual-function vaccine designed to generate antibodies to reduce
Journal of bacteriology, 178(24), 7322-7325 (1996-12-01)
Pseudomonas aeruginosa produced alginate and elevated algD (encoding GDPmannose 6-dehydrogenase) transcription under strict anaerobic conditions, especially when using nitrate as a terminal electron acceptor. Purified alginate added to bacterial suspensions caused a decrease in growth, suggesting that alginate contributes to
Pathogens and disease, 67(3), 159-173 (2013-04-27)
Pseudomonas aeruginosa is a metabolically versatile bacterium that can cause a wide range of severe opportunistic infections in patients with serious underlying medical conditions. These infections are characterized by an intense neutrophilic response resulting in significant damage to host tissues
Cell, 48(1), 129-136 (1987-01-16)
Pseudomonas exotoxin A is a single chain toxin with three structural domains that inhibits protein synthesis in eukaryotic cells by catalyzing ADP ribosylation of elongation factor 2. To study the function of these domains, we deleted different portions of the
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