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SAB4600458

Sigma-Aldrich

Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, CF660C antibody produced in goat

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

goat

결합

CF 660C conjugate

항체 형태

affinity isolated antibody

항체 생산 유형

secondary antibodies

클론

polyclonal

형태

buffered aqueous solution

종 반응성

chicken

농도

~2 mg/mL

기술

flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 1-10 μg/mL
western blot: suitable

형광

λex 667 nm; λem 685 nm

저장 온도

−20°C

타겟 번역 후 변형

unmodified

일반 설명

This product is prepared by labeling highly cross-adsorbed goat anti-chicken IgY (IgG) (H+L) with CF660C. CF660C is a cyanine-based dye engineered to be highly water-soluble without being excessively charged. As a result, CF660C-antibody conjugates have high signal-to-noise ratio in immunostaining. CF660C is several fold brighter and significantly more photostable than Alexa Fluor 660. CF660C absorbs at 667 nm but can still be sufficiently excited by the 633 or 635 nm laser with emission at 685 nm in the borderline spectral region between far-red and near-IR fluorescence. Please also see CF660R, an exceptionally photostable 660 nm dye ideal for confocal microscopy application. To minimize cross-reactivity, the antibody has been adsorbed against Bovine, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat, and Sheep serum. 

특이성

Binds all chicken IgYs (minimal cross-reaction with bovine, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit, rat and sheep proteins)

면역원

chicken IgY (H+L)

특징 및 장점

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

물리적 형태

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.

제조 메모

Protect from light. The antibody solution should be gently mixed before use.

법적 정보

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Leonid Andronov et al.
Communications biology, 5(1), 1100-1100 (2022-10-18)
Single molecule localization microscopy (SMLM) with a dichroic image splitter can provide invaluable multi-color information regarding colocalization of individual molecules, but it often suffers from technical limitations. Classical demixing algorithms tend to give suboptimal results in terms of localization precision
Yongrong Liao et al.
The Journal of cell biology, 223(7) (2024-04-23)
Assembly of macromolecular complexes at correct cellular sites is crucial for cell function. Nuclear pore complexes (NPCs) are large cylindrical assemblies with eightfold rotational symmetry, built through hierarchical binding of nucleoporins (Nups) forming distinct subcomplexes. Here, we uncover a role

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