생물학적 소스
alpaca
재조합
expressed in E. coli
결합
horse radish peroxidase (HRP)
항체 형태
affinity purified immunoglobulin
항체 생산 유형
secondary antibodies
클론
polyclonal
형태
lyophilized solid
분자량
14 kDa
종 반응성
Alpaca, mouse
기술
ELISA: 1:10000-1:50000
western blot: 1:10000-1:50000
크기
0.1 mL
색상
colorless
동형
VHH
배송 상태
wet ice
저장 온도
2-8°C
일반 설명
Alpaca Anti-Mouse IgG(Fcγ) HRP single domain Antibody is designed to specifically detect the Fc region of mouse IgG. It is a single domain recombinant antibody to mouse IgG(Fcγ fragment specific) coupled to HRP. Alpaca Anti-Mouse IgG(Fcγ) HRP single domain Antibody detects the Fc region of mouse IgG selectively but not with mouse IgM, or the Fab portion of mouse immunoglobulins.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
특이성
Mouse IgG(Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c
Isotype: VHH domain of alpaca IgG2b/2c
면역원
Recombinant Alpaca single variable domain secondary nano antibody
포장
100 μg in Glass bottle
물리적 형태
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
재구성
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
저장 및 안정성
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C
기타 정보
No cross-reactivity with mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
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신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
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