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Merck
모든 사진(2)

문서

SAB5801009

Sigma-Aldrich

Alpaca Anti-Mouse IgG2b(Fcγ) single domain Antibody

recombinant Alpaca(VHH) Nano antibody

동의어(들):

Recombinant Alpaca single variable domain secondary Nanobody

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

alpaca

재조합

expressed in E. coli

결합

unconjugated (6*his tag and one cys were added at the C terminal of the VHH)

항체 형태

affinity purified immunoglobulin

항체 생산 유형

secondary antibodies

클론

recombinant monoclonal

형태

lyophilized solid

분자량

14 kDa

종 반응성

Alpaca, mouse

기술

ELISA: 1:10000-1:50000
western blot: 1:10000-1:50000

크기

0.1 mL

색상

colorless

동형

VHH

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

Alpaca Anti-Mouse IgG2b(Fcγ) single domain Antibody is designed to specifically detect mouse IgG2b Fcγ fragment. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse IgG2b(Fcγ) single domain Antibody reacts with the Fc fragment of mouse IgG2b selectively but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

특이성

Mouse IgG2b (Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c

면역원

Recombinant Alpaca single variable domain secondary Nanobody
Recombinant Alpaca single variable domain secondary nano antibody

포장

100 μg in Glass bottle

물리적 형태

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

재구성

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

저장 및 안정성

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

기타 정보

No cross-reactivity with mouse IgG1/2a/3, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG

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픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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