SAE0045

HRV-3C Protease.

N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

• 동의어(들): Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: human (human Rhinovirus Type 14)
• Quality Level: 200
• 분석: ≥90% (SDS-PAGE)
• 형태: aqueous solution
• 특이 활성도: ≥5000 U/mg
• 분자량: 21 kDa
• 농도: 0.8-1.2 mg/mL
• 기술: protein purification: suitable
• 적합성: suitable for protein modification
• 응용 분야: life science and biopharma
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.

애플리케이션

HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein.

단위 정의

One unit of HRV-3C Protease is defined as the amount of enzyme needed to digest 1nmole of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pNA) at 0 °C, in a reaction buffer consisting of 25 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

물리적 형태

Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM TCEP and 50% (V/V) glycerol.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2