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모든 사진(1)

Key Documents

SRP2118

Sigma-Aldrich

RNA Polymerase II, RPB10 subunit, GST tagged human

recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

동의어(들):

RBP10, RPABC5, RPB10, RPB10beta, RPB7.6, hRPB7.6, hsRPB10b

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About This Item

UNSPSC 코드:
12352202
NACRES:
NA.26

생물학적 소스

human

재조합

expressed in E. coli

분석

≥85% (SDS-PAGE)

형태

frozen liquid

분자량

~33.6 kDa

포장

pkg of 10 μg

저장 조건

avoid repeated freeze/thaw cycles

농도

300 μg/mL

색상

clear colorless

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−70°C

유전자 정보

human ... POLR2L(5441)

생화학적/생리학적 작용

The RNA polymerase subunit RPB10 displays a high level of conservation across archaea and eukarya and is required for cell viability in yeast. It is a zinc-binding protein with an atypical CX2CXnCC metal binding motif. RPB10 participates in protein-protein interactions with additional components of the polymerase holoenzyme. Experimentally, the binding of RPB10 to an RPB3-RPB11 (or RPAC40-RPAC19 in RNAPI/III) heterodimer is well characterized and persists in both eukaryal and archaeal RNAPs. Binding of RPB10 to this a2-like heterodimer through zinc-mediated hemi-coordination suggests an early role in holoenzyme assembly since the formation of the α2-complex is the first step in the assembly of the prokaryotic RNAPs.

물리적 형태

Clear and colorless frozen liquid solution

제조 메모

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

N A Woychik et al.
Trends in biochemical sciences, 15(9), 347-351 (1990-09-01)
RNA polymerase II is the core of the complex apparatus that is responsible for the regulated synthesis of mRNA. A comprehensive knowledge of RNA polymerase II is essential to our understanding of the molecular mechanisms through which a variety of
C D Mackereth et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(12), 6316-6321 (2000-06-07)
The RNA polymerase subunit RPB10 displays a high level of conservation across archaea and eukarya and is required for cell viability in yeast. Structure determination of this RNA polymerase subunit from Methanobacterium thermoautotrophicum reveals a topology, which we term a
N A Woychik et al.
Genes & development, 4(3), 313-323 (1990-03-01)
RNA polymerases I, II, and III share three subunits that are immunologically and biochemically indistinguishable. The Saccharomyces cerevisiae genes that encode these subunits (RPB5, RPB6, and RPB8) were isolated and sequenced, and their transcriptional start sites were deduced. RPB5 encodes

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