T0910

Thioredoxin from Escherichia coli

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

• CAS Number: 52500-60-4
• MDL number: MFCD00132423
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: Escherichia coli
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: ≥90% (SDS-PAGE)
• 형태: essentially salt-free, lyophilized powder
• 특이 활성도: ≥3 units/mg protein
• 분자량: 11.7 kDa
• 기술: activity assay: suitable
• solubility: water: 1.90-2.10 mg/mL, clear, colorless
• 단백질 ID 수납 번호: M26133
• UniProt 수납 번호: P0AA25
• 응용 분야: cell analysis
• 저장 온도: −20°C
• 유전자 정보: Escherichia coli K12 ... trxA(948289)

설명

일반 설명

Research area: Cell Signaling

Thioredoxin from Escherichia coli is a monomeric, globular protein. It has a central β-sheet surrounded by α-helices.

애플리케이션

Thioredoxin from Escherichia coli has been used in thioredoxin reductase assay, peroxidase-coupled thioredoxin system assay and peroxiredoxin assay. It has also been used to study its effects on photoreceptor death in a mouse model of retinitis pigmentosa (RP) and its potential to modify retinal oxidative stress, glial changes, and vascular changes during the progression of the disease.

생화학적/생리학적 작용

Thioredoxin from Escherichia coli catalyzes REDOX reactions and has a role in various biological processes. It modulates the structure and activity of many proteins. The enzyme thioredoxin reductase catalyzes the reduction of thioredoxin. Thioredoxin also has a role in oxidative stress response.Thioredoxin, known as both adult T cell leukemia-derived factor (ADF) and a cellular redox enzyme, functions as a cytokine or chemokine, facilitating cell-to-cell communication. Additionally, it plays a role in regulating cell growth and apoptosis.

Mediates the reduction of disulfide bonds in proteins.

기타 정보

GenBank^® Accession Number M26133.

단위 정의

One unit will cause a ΔA₆₅₀ of 1.0 in 1 min at 25 °C in the insulin reduction assay.

법적 정보

GenBank is a registered trademark of United States Department of Health and Human Services

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)