T6025

Tris Acetate-EDTA buffer

BioReagent, suitable for electrophoresis

• 동의어(들): TAE buffer
• MDL number: MFCD00236357
• UNSPSC 코드: 41105319
• PubChem Substance ID: 329826837
• NACRES: NA.25

속성

• 무균: 0.2 μm filtered
• Quality Level: 200
• 제품 라인: BioReagent
• 형태: working solution
• 기술: electrophoresis: suitable
• 불순물: DNase, RNase, Protease, none detected
• 적합성: suitable for electrophoresis; suitable for gel electrophoresis (after dilution to working concentration)
• 응용 분야: diagnostic assay manufacturing
• SMILES string: CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
• InChI key: HGEVZDLYZYVYHD-UHFFFAOYSA-N

설명

애플리케이션

Tris Acetate-EDTA buffer has been used as run buffer for the agarose gel electrophoresis of human papillomavirus DNA and transcribed RNA samples.

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

기타 정보

40 mM Tris acetate, pH approx. 8.3, containing 1 mM EDTA.

제조 메모

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Solution prepared with 18 megohm water

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)