추천 제품
형태
lyophilized powder
특이 활성도
≥60 NIH units/mg protein (biuret)
구성
Protein, ≥50%
저장 온도
−20°C
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일반 설명
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Thrombin comprises of A-chain and B-chain with the catalytic pocket. The C-terminal regions has a fibrinogen recognition site (FRS) and a heparin binding site (HBS). The main function of thrombin is cleavage of fibrinogen to fibrin. It coordinates the coagulation cascade and coordinates with protease-activated receptors (PARs) to regulate physiological functions. High levels of thrombin causes neurotoxicity in dopaminergic neurons and contribute to the progression of parkinsons disease. Altered thrombin levels modulates coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin.
애플리케이션
Thrombin from bovine plasma may be used:
as revitalization model to mimic blood fabrication in root canal
- for the generation of fibrin from fibrinogen embedded in microbubbles
- for the cleavage of hydrogenase maturation protein (HypF-N) from the nickel resin
as revitalization model to mimic blood fabrication in root canal
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to evaluate coagulation abnormalities in acute liver failure.
생화학적/생리학적 작용
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
단위 정의
Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.
물리적 형태
Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0
제조 메모
Traditional thrombin products are activated with bovine brain thromboplastin, whereas T7326 is activated with bovine lung thromboplastin and does not contain any bovine brain products in its preparation.
분석 메모
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
기타 정보
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Fibrin-Targeted Polymerized Shell Microbubbles as Potential Theranostic Agents for Surgical Adhesions
Gormley CA, et al.
Langmuir (2019)
Backbone NMR assignments of HypF-N under conditions generating toxic and non-toxic oligomers
Patel JR, et al.
Biomolecular NMR Assignments, 1-5 (2018)
Banwari Agarwal et al.
Journal of hepatology, 57(4), 780-786 (2012-06-28)
In acute liver failure (ALF), prothrombin time (PT) and its derivative prothrombin time ratio (PTR) are elevated, and are considered predictors of increased bleeding risk. We aimed at determining whether increased PT/PTR reflects the haemostatic potential and bleeding risk in
Increased plasma thrombin potential is associated with stable coronary artery disease: An angiographically-controlled study
Tosi F, et al.
Thrombosis Research, 155, 16-22 (2017)
Thrombin generation correlates with disease duration in multiple sclerosis (MS): Novel insights into the MS-associated prothrombotic state
Parsons MEM, et al.
Multiple Sclerosis Journal, 3(4), 84-84 (2017)
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