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Merck
모든 사진(3)

주요 문서

XNAP2

Sigma-Aldrich

Extract-N-Amp Plant PCR Kit

sufficient for 100 extractions, sufficient for 100 amplifications

동의어(들):

Plant direct PCR kit

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About This Item

UNSPSC 코드:
41106303
NACRES:
NA.55

사용

sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions

Quality Level

특징

dNTPs included
hotstart

기술

PCR: suitable

색상

colorless

응용 분야

agriculture

배송 상태

wet ice

저장 온도

−20°C

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일반 설명

The Extract-N-Amp Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.

애플리케이션

Extract-N-Amp Plant PCR Kit has been used for genomic DNA isolation from plants and the fungal mycelium. It has also been used in polymerase chain reaction (PCR).
Extract-N-Amp Plant PCR Kit is suitable for use in genotyping.

It has been used forDNA extraction from fresh mushrooms.

특징 및 장점

  • Plant genomic DNA can be extracted for PCR in under 15 minutes using a method.
  • No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary
  • A specially formulated PCR ReadyMix is provided for use with the extracted DNA
  • The Hot Start antibody ensures highly specific PCR amplification of genomic DNA
  • Suitable for high-throughput plant genetic analysis.
  • Lengthy enzymatic digestions are not required, saving time and effort.
  • The extracted DNA remains stable at 4 °C for atleast 6 months.

성분

Extract-N-Amp Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)

기타 정보

For additional information, please see www.sigma-aldrich.com/extract-n-amp.
The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.

법적 정보

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

Lot/Batch Number

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Elin Videvall et al.
mSystems, 2(6) (2017-11-29)
The gut microbiome of animals is emerging as an important factor influencing ecological and evolutionary processes. A major bottleneck in obtaining microbiome data from large numbers of samples is the time-consuming laboratory procedures required, specifically the isolation of DNA and
Ante Karoglan et al.
Acta dermato-venereologica, 99(13), 1253-1257 (2019-10-02)
Imbalance in skin microflora, particularly related to certain Cutibacterium acnes strains, may trigger acne. Application of non-acne-causing strains to the skin may modulate the skin microbiome and thereby lead to a reduction in acne. This pilot study evaluates the safety
Phylogenetic placements and cultural characteristics of Tuber species isolated from ectomycorrhizas
Obase K, et al.
Mycoscience (2021)
Len N Gillman et al.
Journal of evolutionary biology, 23(6), 1327-1330 (2010-05-12)
A faster rate of nuclear DNA evolution has recently been found for plants occupying warmer low latitudes relative to those in cooler high latitudes. That earlier study by our research group compared substitution rates within the variable internal transcribed spacer
Dionne N Shepherd et al.
Journal of virological methods, 149(1), 97-102 (2008-02-19)
A high-throughput method of isolating and cloning geminivirus genomes from dried plant material, by combining an Extract-n-Amp-based DNA isolation technique with rolling circle amplification (RCA) of viral DNA, is presented. Using this method an attempt was made to isolate and

문서

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

프로토콜

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

관련 콘텐츠

Explore technology and reagent portfolios for plant breeding workflows, accelerating the development of new crop varieties.

Learn about our broad technology and reagent portfolios to help you with the discovery, development, and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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