Y2001

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, tryptophan and uracil

• 동의어(들): Synthetic drop-out medium for yeast
• MDL number: MFCD01095679
• UNSPSC 코드: 12000000
• NACRES: ND.02

속성

• Quality Level: 100
• 양식: powder
• 응용 분야: food and beverages

설명

애플리케이션

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

성분

The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for histidine, leucine, tryptophan and uracil. Each amino acid is present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L)

기타 정보

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

수량

1.4 g will supplement 1 liter of medium.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)