ZRB1595

Anti-IKKβ Antibody, clone 2N22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 동의어(들): Inhibitor of nuclear factor kappa-B kinase subunit beta;EC:2.7.11.10;I-kappa-B-kinase beta;IKK-B;IKK-beta;IkBKB;I-kappa-B kinase 2;IKK2;Nuclear factor NF-kappa-B inhibitor kinase beta;NFKBIKB;Serine/threonine protein kinase IKBKB
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 재조합: expressed in HEK 293 cells
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 2N22, recombinant monoclonal
• 설명: recombinant, expressed in HEK 293 cells
• 제품 라인: ZooMAb^® learn more
• 형태: lyophilized
• 분자량: calculated mol wt 86.56 kDa; observed mol wt ~90 kDa
• 정제법: using Protein A
• 종 반응성: mouse, rat, human
• 종 반응성(상동성에 의해 예측): bovine, monkey
• 포장: antibody small pack of 25 μL
• 환경친화적 대안 제품 특성: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 기술: ELISA: suitable; affinity binding assay: suitable; western blot: suitable
• 동형: IgG
• 에피토프 서열: N-terminal half
• 단백질 ID 수납 번호: NP_001177649
• UniProt 수납 번호: O14920
• 환경친화적 대안 카테고리: Aligned,
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

특이성

Clone 2N22 is a Rabbit recombinant monoclonal antibody that specifically detects IKKb. It targets an epitope within 24 amino acids from the N-terminal half.

면역원

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal half of human IKKb.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IKKβ in A549 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IKKβ in HepG2, L6, and NIH3T3 cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound IKKb with a KD of 7.9 x 10-8 in an affinity binding assay.

Enzyme Immunoassay (ELISA) Analysis: Various dilutions of this antibody detected IKKβ in ELISA application.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

표적 설명

Inhibitor of nuclear factor kappa-B kinase subunit beta (UniProt: O14920; also known as EC:2.7.11.10, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, Serine/threonine protein kinase IKBKB) is encoded by the IKBKB (also known as IKKB) gene (Gene ID: 3551) in human. The IKK complex is composed of three subunits, IKKa , IKKb, and IKKg (NEMO). While IKKa and IKKb are highly similar catalytic subunits and can phosphorylate IkB, IKKg is the regulatory subunit. IKKb is a serine kinase that plays an essential role in the NF-kB signaling pathway. It acts as a part of the canonical IKK complex in the conventional pathway of NF-kB activation. Its protein kinase domain is localized in amino acids 15-300. It phosphorylates inhibitors of NF-kB (IkB) on two critical serine residues that allow polyubiquitination of these inhibitors and their subsequent degradation by the proteasome. This allows free NF-kB to translocate into the nucleus and activate the transcription of genes involved in immune response, growth control, and protection against apoptosis. Upon cytokine stimulation, IKKb is phosphorylated on serine 177 and serine 181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and protein kinase Cz that enhances its activity. Once activated, it undergoes autophosphorylation on the C-terminal serine cluster, which reduces its activity and prevents prolonged activation of the inflammatory response. Mutations in IKBKB gene are known to cause life threatening immunodeficiency that develops in in infancy and increases susceptibility to bacterial, fungal, and viral infections. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, ZW., et al. (1999). J. Exp. Med. 189(11); 1839-1845).

물리적 형태

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

재구성

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

법적 정보

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable