생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
1O17, recombinant monoclonal
설명
recombinant, expressed in HEK 293 cells
제품 라인
ZooMAb® learn more
양식
lyophilized
분자량
calculated mol wt 22.78 kDa
observed mol wt ~30 kDa
정제법
using Protein A
종 반응성
human
종 반응성(상동성에 의해 예측)
bovine, horse, mouse, feline, monkey, canine, rat
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
동형
IgG
에피토프 서열
N-terminal half
단백질 ID 수납 번호
UniProt 수납 번호
환경친화적 대안 카테고리
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
phosphorylation (pSer82)
유전자 정보
human ... HSPB1(3315)
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
특이성
Clone 1O17 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects HSP27 phosphorylated on serine 82.
면역원
KLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphoserine 82 from the N-terminal half of human Heat shock protein 27 (HSP27).
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in lysates from HeLa cells overnight serum starved and treated with Anisomycin.
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected HSP27 phosphorylated on serine 82 in lysates of HeLa cells overnight serum starved and treated with Anisomycin (25 μg/mL; 30 min.).
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected phospho-HSP27 Ser82 in lysate from UV-treated HeLa cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-HSP27 Ser82 in human small intestine and human colon cancer tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound phospho-HSP27-ser82 peptide with a KD of 1. x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-HSP27 Ser82 in HeLa cells treated with Anisomycin.
Peptide Inhibition Assay: Target band detection in lysate from HeLa cells treated with Anisomycin was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysates from HeLa cells overnight serum starved and treated with Anisomycin.
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected HSP27 phosphorylated on serine 82 in lysates of HeLa cells overnight serum starved and treated with Anisomycin (25 μg/mL; 30 min.).
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected phospho-HSP27 Ser82 in lysate from UV-treated HeLa cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-HSP27 Ser82 in human small intestine and human colon cancer tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound phospho-HSP27-ser82 peptide with a KD of 1. x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-HSP27 Ser82 in HeLa cells treated with Anisomycin.
Peptide Inhibition Assay: Target band detection in lysate from HeLa cells treated with Anisomycin was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
표적 설명
Heat shock protein beta-1 (UniProt: P04792; also known as HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27) is encoded by the HSPB1 (also known as HSP27, HSP28) gene (Gene ID: 3315) in human. Heat shock proteins (HSPs) are produced during nonlethal stress conditions and protect the organism from severe and sometimes lethal stress. HSPs can also be induced by non-heat related stress factors. HSP27 is a multidimensional protein that acts as a protein chaperone and an antioxidant and plays a role in the inhibition of apoptosis and actin cytoskeletal remodeling. It is detected in all tissues; however, its highest levels are found in the heart and in tissues composed of striated and smooth muscle. It is shown to be mostly cytoplasmic in interphase cells and is shown to colocalize with mitotic spindles in mitotic cells. It translocates to the nucleus during heat shock and resides in sub-nuclear structures known as nuclear splicing speckles and functions to stabilize DNA and/or the nuclear membrane. HSP-27 contains a conserved -crystalline homology domain at its C-terminal. It is phosphorylated upon exposure to protein kinase C activators and heat shock. HSP27 is present at basal levels in cells and tissues and is organized as large oligomers. After heat shock, exposure to chemical stress, or addition of steroid hormones, its synthesis increases several fold. It is phosphorylated by MAPKAP kinase 2/3 via the activation of the p38 MAPK pathway at multiple serine residues. Following phosphorylation, HSP27 reorganizes itself into smaller oligomers that interact with other proteins. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Alderson TR., et al. (2019). Nat. Commun. 10; Article 1068; Vidyasagar, A., et al. (2012). Fibrogenesis Tissue Repair. 5(1); 7; Landry, J., et al. (1992). J. Biol. Chem. 267(2); 794-803).
물리적 형태
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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