기술
HPLC: suitable
검사 파라미터
column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] water; [B] methanol; [C] acetonitrile; (720:120:120, A:B:C) containing 0.780 g/L potassium bromide, 230 μL/L nitric acid
flow rate: 0.400 mL/min
pressure: 4568 psi (315 bar)
column temp.: 35 °C
detector: FLD excitation = 360 nm, emission = 440 nm
injection: 20 μL
sample: 200 μL spiked 84:16 acetonitrile:di water in 880 μL di water, Spike concentrations: B1 = 16 ppb; B2 = 4 ppb; G1 = 16 ppb; B2 = 4 ppb
적합성
application for HPLC
응용 분야
food and beverages
분석 메모
Aflatoxins are a complex of closely related toxins produced as secondary metablolites of fungi. They are found in grains and nuts such as corn, wheat, or peanuts. They are produced by aspergillus flavus and a. Parasiticus. Members of the group include aflatoxin b1, aflatoxin b2, aflatoxin g1, aflatoxin g2, aflatoxin m1, and aflatoxin m2.
법적 정보
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
분석물
설명
Aflatoxin G2
Aflatoxin G1
Aflatoxin B2
Aflatoxin B1
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