E5134

Ethylenediaminetetraacetic acid disodium salt dihydrate

suitable for electrophoresis, Molecular Biology, 99.0-101.0% (titration)

• Synonym(s): Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na₂, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na₂, Sequestrene Na₂
• สูตรเอมพิริกัล (Hill Notation): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
• CASE番号: 6381-92-6
• น้ำหนักโมเลกุล: 372.24
• NACRES: NA.31
• PubChem Substance ID: 24894605
• UNSPSC Code: 12352107
• EC Number: 205-358-3
• Beilstein/REAXYS Number: 3900609

คุณสมบัติ

• grade: Molecular Biology
• Quality Level: 300
• assay: 99.0-101.0% (titration)
• form: powder
• reaction suitability: reagent type: chelator
• technique(s): electrophoresis: suitable
• impurities: ≤0.005% Insolubles, ≤0.1% Nitrilotriacetic acid (NTA)
• mp: 248 °C (dec.) (lit.)
• solubility: 3 M NaOH: 1.6 g/10 mL
• cation traces: Fe: ≤0.005%, Pb: ≤0.002%
• suitability: suitable for electrophoresis, suitable for molecular biology
• foreign activity: DNase, RNase, NICKase and protease, none detected
• storage temp.: room temp
• SMILES string: O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
• InChI key: OVBJJZOQPCKUOR-UHFFFAOYSA-L

คำอธิบาย

General description

EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests

Application

EDTA disodium salt has been used in research:

• as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more
• an anticoagulant for whole blood collection
• a stabilizer solution for freezing cell culture samples
• in combination with PBS and trypsin, to trypsinize cells
• for phytoextraction of heavy metals in polluted soils

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Features and Benefits

• Molecular Biology grade, suitable for many molecular biology applications
• Free of endonucleases DNase, RNase, Nickase, and Protease

Other Notes

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ข้อมูลความปลอดภัย

• pictograms: GHS07,GHS08
• signalword: Warning
• hcodes: H332 - H373
• pcodes: P260 - P271 - P304 + P340 + P312 - P314 - P501
• Hazard Classifications: Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
• target_organs: Respiratory Tract
• คลาสการจัดเก็บ: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)