Small molecule method development and analysis which includes medicinal chemistry, metabolite identification, impurity profiling, drug metabolism and pharmacokinetics, are key to the success of pharmaceutical quality control and assurance. Successful testing and release of small molecule drugs is dependent on efficient analytical tools, validation guidelines and practices.
There are several methods available for the separation of small molecules and their identification. Some include wet chemistry, high performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), capillary electrophoresis (CE), nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS).
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