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S9007

Sigma-Aldrich

Spectinomycin dihydrochloride pentahydrate

potency: ≥603 μg per mg

Synonym(s):

Actinospectacin, Actinospectacin dihydrochloride pentahydrate, Spectinomycin, Spectinomycin HCL, Spectinomycin Hydrochloride, Spectinomycin hydrochloride

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100 MG
$128.00
500 MG
$372.00
1 G
$642.00
5 G
$2,710.00

$128.00

Available to ship onApril 25, 2025Details

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100 MG
$128.00
500 MG
$372.00
1 G
$642.00
5 G
$2,710.00

About This Item

Empirical Formula (Hill Notation):
C14H24N2O7 · 2HCl · 5H2O
CAS Number:
22189-32-8
Molecular Weight:
495.35
EC Number:
244-554-3
MDL number:
MFCD00150886
UNSPSC Code:
51281666
PubChem Substance ID:
24899822
NACRES:
NA.85

$128.00

Available to ship onApril 25, 2025Details

Request a Bulk Order

biological source

synthetic (inorganic)

Quality Level

200

form

powder

potency

≥603 μg per mg

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

color

white to pale buff

solubility

H2O: soluble 50 mg/mL

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

agriculture

mode of action

protein synthesis | interferes

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This Item
C6885C1889C1764
Collagenase from Clostridium histolyticum suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV), Type VIII, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

C2139

Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum suitable for release of physiologically active rat epididymal adipocytes, Type II, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

C6885

Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum 0.2 μm filtered, suitable for release of physiologically active rat hepatocytes, Type IV-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

C1889

Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum 0.2 μm filtered, suitable for release of physiologically active rat epididymal adipocytes, Type II-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

C1764

Collagenase from Clostridium histolyticum

specific activity

≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid

specific activity

≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid

specific activity

≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid

specific activity

≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid

biological source

Clostridium histolyticum

biological source

Clostridium histolyticum

biological source

-

biological source

-

form

powder

form

powder

form

powder

form

lyophilized powder

suitability

suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV)

suitability

-

suitability

suitable for release of physiologically active rat hepatocytes

suitability

suitable for release of physiologically active rat epididymal adipocytes

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

mol wt

68-125 kDa

mol wt

-

mol wt

68-130 kDa

mol wt

68-125 kDa

General description

Chemical structure: aminoglycoside

Application

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

Biochem/physiol Actions

Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.

Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).

Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.

Caution

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Preparation Note

Stock solutions should be prepared at 10 mg/mL in water and filter sterilized.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000403487
    0000403485
    0000337404
    0000337404
    0000403487

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    Stefan Schwarz et al.
    The Journal of antimicrobial chemotherapy, 53(2), 379-382 (2004-01-20)
    Prior to the renewal of spectinomycin licensing for veterinary uses in Germany, 154 Pasteurella multocida and 148 Mannheimia haemolytica strains from respiratory tract infections in cattle were investigated for their MICs of spectinomycin and other antimicrobial agents. The data obtained
    Amber M Hotto et al.
    G3 (Bethesda, Md.), 1(7), 559-570 (2012-03-03)
    Noncoding RNAs (ncRNA) are widely expressed in both prokaryotes and eukaryotes. Eukaryotic ncRNAs are commonly micro- and small-interfering RNAs (18-25 nt) involved in posttranscriptional gene silencing, whereas prokaryotic ncRNAs vary in size and are involved in various aspects of gene
    Tarinee Tungsuchat-Huang et al.
    Plant molecular biology, 76(3-5), 453-461 (2011-01-05)
    Identification of a genetically stable Nicotiana tabacum (tobacco) plant with a uniform population of transformed plastid genomes (ptDNA) takes two cycles of plant regeneration from chimeric leaves and analysis of multiple shoots by Southern probing in each cycle. Visual detection
    Liu-Feng Yuan et al.
    Sexually transmitted diseases, 38(8), 764-768 (2011-08-17)
    Antimicrobial resistance in Neisseria gonorrhoeae (NG) is a public health concern globally, and azithromycin-resistant NG isolates have been identified in many countries. This study aimed to investigate azithromycin susceptibility in NG and to genetically characterize the azithromycin-resistant isolates identified in
    Xuguang Jiang et al.
    Environmental science & technology, 46(24), 13539-13544 (2012-11-21)
    With fast development of industry large quantities of hazardous waste are produced in China. Today, incineration plays an important role in the disposal of hazardous waste. Co-incineration of some types of hazardous wastes with municipal solid waste (MSW) has been
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