HYASKIT
HyasKit
Synonym(s):
Microenvironment mapping technology, Photoinduced proximity labeling kit, Protein-protein interaction elucidation technology, Small molecule-photocatalyst conjugate labeling
About This Item
Recommended Products
storage temp.
−20°C
General description
The kit provides a step-by-step procedure for conjugating your ligand of choice to a photocatalyst that localizes light-mediated crosslinking to the ligands target. The ligand–iridium conjugate can then be incubated with live cells along with a biotinylated diazirine reagent. Analogous to a satellite, the photocatalyst localizes carbene generation from the diazirine only in the direct vicinity and thus covalently modifies the target protein in a catalytic manner, amplifying the signal for downstream analysis.
To link to an acid on a small molecule (instead of an amine), the Dione Kit is available.
Photoreactors commercially available:
- Penn PhD Photoreactor M2 (Z744035)
- Penn PhD Photoreactor M2 365nm Light Source (Z744031)
- LightOX PhotoReact 365 (Z744061)
Read more about: - Target identification with HyasKit and DioneKit in our Technology Spotlight
- Small molecule photocatalysis enables drug target identification via energy transfer
Application
Biochem/physiol Actions
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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Articles
In cases where photoaffinity labeling (PAL) probes are ineffective, Hyas and Dione Kits provide an effective method for small molecule identification by using an Iridium photocatalyst tethered to the molecule under investigation.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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