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L030004

Sigma-Aldrich

ETT Activator

0.25M

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Synonym(s):
5-(Ethylthio)-1H-tetrazole, 5-(Ethylthio)tetrazole solution
MDL number:

Quality Level

type

for DNA synthesis

product line

Proligo Reagents

form

liquid

concentration

32-35 g/L (titration)

impurities

≤30 ppm water content (Karl Fischer)

SMILES string

CCSc1nnn[nH]1

InChI

1S/C3H6N4S/c1-2-8-3-4-6-7-5-3/h2H2,1H3,(H,4,5,6,7)

InChI key

GONFBOIJNUKKST-UHFFFAOYSA-N

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Related Categories

Preparation Note

Acetonitrile / 5-(Ethylthio)-1H-Tetrazole 100 / 3 (v/w)

Other Notes

Activator solution, containing 5-(Ethylthio)-1H- tetrazole (ETT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

35.6 °F - closed cup

flash_point_c

2.0 °C - closed cup


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