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04-1585

Sigma-Aldrich

Anti-IL-18 Antibody, clone 12E7.1

clone 12E7.1, from mouse

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Synonym(s):
interleukin 18 (interferon-gamma-inducing factor), IL-1 gamma, IFN-gamma-inducing factor, iboctadekin, interleukin-18, Interleukin-1 gamma, Iboctadekin, Interferon gamma-inducing factor
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

12E7.1, monoclonal

species reactivity

human, mouse

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable

isotype

IgMκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... IL18(3606)
mouse ... Il18(16173)

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HPA003980ABS433SAB2501258
vibrant-m

04-1585

Anti-IL-18 Antibody, clone 12E7.1

vibrant-m

ABS433

Anti-IL-32 Antibody

species reactivity

human, mouse

species reactivity

human

species reactivity

human

species reactivity

human

clone

12E7.1, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

goat

Gene Information

human ... IL18(3606)
mouse ... Il18(16173)

Gene Information

human ... IL18(3606)

Gene Information

human ... IL32(9235)

Gene Information

human ... IL18(3606)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

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General description

Interleukin-18 (UniProt: Q14116; also known as IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma) is encoded by the IL18 (also known as IGIF, IL1F4) gene (Gene ID: 3606) in human. IL-18 is a pro-inflammatory cytokine that is shown to evoke both innate and acquired responses. It is synthesized with a propeptide (aa 1-36), which is subsequently cleaved off by caspases 1 and 4 to generate the active mature form. IL-18 is reported to be constitutively expressed in monocytes, macrophages, epithelial cells, osteoblasts, keratinocytes, and synovial fibroblasts. Its expression has also been reported in adrenal cortical cells. Mature IL-18 acts by binding to the IL-18 receptor α (IL-18α) to form a low affinity binary complex that recruits IL-18β to form a high affinity complex that leads to the activation of NF-kB and MAP kinase pathways. IL-18 can synergize with IL-12 to induce interferon-γ synthesis from T-helper 1 and natural killer cells.

Immunogen

KLH-conjugated linear peptide corresponding to human IL-18.

Application

Anti-IL-18 Antibody, clone 12E7.1 is an antibody against IL-18 for use in IH, FC.
Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in human tonsil tissue.

Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in inflamed skin and normal human spleen tissues.
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors

Quality

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 1 g of this antibody detected IL-18 in mouse splenocytes.

Target description

~24 kDa observed

Physical form

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse splenocytes

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Zhaoxia Liang et al.
Journal of neuroinflammation, 16(1), 226-226 (2019-11-18)
Chemerin is highly expressed in the serum, placenta tissue, and umbilical cord blood of diabetic mother; however, the impact of chemerin on cognitive disorders of offspring from mothers with diabetes in pregnancy remains unclear. A diabetic phenotype in pregnant mice
Solène Gatault et al.
Journal of immunology (Baltimore, Md. : 1950), 195(5), 2483-2492 (2015-07-29)
Eosinophils are multifunctional leukocytes that are involved in innate and adaptive immune responses through the expression of various receptors and mediators. Previously, we showed that human eosinophils and T cells shared cytotoxic activities against tumor cells that involved the γ-δ
Hung-Yu Chiang et al.
Nature communications, 13(1), 874-874 (2022-02-17)
IL-18 is emerging as an IL-22-induced and epithelium-derived cytokine which contributes to host defence against intestinal infection and inflammation. In contrast to its known role in Goblet cells, regulation of barrier function at the molecular level by IL-18 is much

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