05-592
Anti-PP2A Antibody, B subunit, clone 2G9
ascites fluid, clone 2G9, Upstate®
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
2G9, monoclonal
species reactivity
rat, bovine, mouse, Xenopus, rabbit, pig, human
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
human ... PPP2CA(5515)
Specificity
PP2A B subunit, isoform reactivity is α > δ,β > γ
Immunogen
Peptide corresponding to residues 398-411 of human PP2A, B subunit
Application
Detect PP2A using this Anti-PP2A Antibody, B subunit, clone 2G9 validated for use in IH, IP & WB.
Research Category
Signaling
Neuroscience
Signaling
Neuroscience
Research Sub Category
Kinases & Phosphatases
Neurodegenerative Diseases
Kinases & Phosphatases
Neurodegenerative Diseases
Quality
routinely evaluated by immunoblot on RIPA lysates from non-stimulated A431 cells or mouse 3T3/A31 cells
Target description
55kDa
Physical form
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide and glycerol to 30%
Ascites
Storage and Stability
2 years at -20°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ilker K Sariyer et al.
Virology, 375(2), 464-479 (2008-03-21)
Previous studies have demonstrated that the JC virus (JCV) late regulatory protein agnoprotein is phosphorylated by the serine/threonine-specific protein kinase-C (PKC) and mutants of this protein at the PKC phosphorylation sites exhibit defects in the viral replication cycle. We have
Jennifer B Jackson et al.
Neoplasia (New York, N.Y.), 14(7), 585-599 (2012-08-21)
Heterotrimeric protein phosphatase 2A (PP2A) consists of catalytic C (PP2Ac), structural A, and regulatory B-type subunits, and its dysfunction has been linked to cancer. Reversible methylation of PP2Ac by leucine carboxyl methyltransferase 1 (LCMT-1) and protein phosphatase methylesterase 1 (PME-1)
Jocelyn A Lee et al.
The Journal of biological chemistry, 293(25), 9636-9650 (2018-05-08)
Leucine carboxyl methyltransferase-1 (LCMT-1) methylates the C-terminal leucine α-carboxyl group of the catalytic subunits of the protein phosphatase 2A (PP2A) subfamily of protein phosphatases, PP2Ac, PP4c, and PP6c. LCMT-1 differentially regulates the formation and function of a subset of the
Chian Ju Jong et al.
The Journal of biological chemistry, 295(17), 5654-5668 (2020-03-12)
Protein phosphatase 2A (PP2A) is a large enzyme family responsible for most cellular Ser/Thr dephosphorylation events. PP2A substrate specificity, localization, and regulation by second messengers rely on more than a dozen regulatory subunits (including B/R2, B'/R5, and B″/R3), which form
Protein phosphatase 2A regulates estrogen receptor alpha (ER) expression through modulation of ER mRNA stability.
Keen, JC; Zhou, Q; Park, BH; Pettit, C; Mack, KM; Blair, B; Brenner, K; Davidson, NE
The Journal of Biological Chemistry null
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