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05-637

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Anti-Bmi-1 Antibody, clone F6

clone F6, Upstate®, from mouse

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Synonym(s):
Polycomb group RING finger protein 4, RING finger protein 51
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

F6, monoclonal

species reactivity

rat, human, mouse, rabbit

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... BMI1(648)

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05-637-AF48805-132105-1362
vibrant-m

05-637

Anti-Bmi-1 Antibody, clone F6

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05-1321

Anti-BMI1 Antibody, clone AF27

vibrant-m

05-1362

Anti-RING1 Antibody, clone 4D6

clone

F6, monoclonal

clone

F6, monoclonal

clone

AF27, monoclonal

clone

4D6, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

Gene Information

human ... BMI1(648)

Gene Information

human ... BMI1(648)

Gene Information

human ... BMI1(648)

Gene Information

human ... RING1(6015)

UniProt accession no.

P25916, P35226

UniProt accession no.

P25916

UniProt accession no.

P35226

UniProt accession no.

Q06587

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General description

The Bmi1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors.
Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Indeed, based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer.

Specificity

Recognizes Bmi-1 (triplet).

Immunogen

Immunized with recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.

Application

Anti-Bmi-1 Antibody, clone F6 is a Mouse Monoclonal Antibody for detection of Bmi-1 also known as Polycomb group RING finger protein 4, RING finger protein 51 and has been validated in ICC, IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Quality

routinely evaluated by immunoblot on RIPA lysates from U2OS cells.

Target description

40-44 kDa

Physical form

Format: Purified
Immunoaffinity Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH 7.4.
Protein G Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
U2OS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos

Other Notes

Please refer to lot specific datasheet.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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John K Mich et al.
eLife, 3, e02669-e02669 (2014-05-21)
Neurosphere formation is commonly used as a surrogate for neural stem cell (NSC) function but the relationship between neurosphere-initiating cells (NICs) and NSCs remains unclear. We prospectively identified, and isolated by flow cytometry, adult mouse lateral ventricle subventricular zone (SVZ)
Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity.
Motoaki Ohtsubo,
Proceedings of the National Academy of Sciences of the USA null
Bmi-1 is a novel molecular marker of nasopharyngeal carcinoma progression and immortalizes primary human nasopharyngeal epithelial cells
Song, Li-Bing, et al
Cancer Research, 66, 6225-6232 (2006)
Inflammation increases cells expressing ZSCAN4 and progenitor cell markers in the adult pancreas.
Ko, SB; Azuma, S; Yokoyama, Y; Yamamoto, A; Kyokane, K; Niida, S; Ishiguro, H; Ko, MS
American Journal of Physiology: Gastrointestinal and Liver Physiology null
Vein tissue expression of matrix metalloproteinase as biomarker for hemodialysis arteriovenous fistula maturation.
Lee ES, Shen Q, Pitts RL, Guo M, Wu MH, Yuan SY
Vascular and Endovascular Surgery null

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