06-1433
Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Basic fibroblast growth factor receptor 1, FGFR-1, bFGF-R-1, Fms-like tyrosine kinase 2, FLT-2, Proto-oncogene c-Fgr, CD331
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
packaging
antibody small pack of 25 μg
technique(s)
cell based assay: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
phosphorylation (pTyr653/pTyr654)
Gene Information
human ... FGFR1(2260)
General description
FGFR-1 (fibroblast growth factor receptor 1) is a member of the basic fibroblast growth factor family of proteins and is thought to be important in the induction and propagation of benign prostatic hyperplasia. A shortage of FGFR-1 causes death post-implantation and has been linked to neurofascin and neurite outgrowth. FGFR-1 expression has recently been found to be increased in patients with renal cell carcinoma and FGFR-1 may also have a role to play in early limb formation.
Specificity
This antibody recognizes FGFR-1 phosphorylated at Tyr653/Tyr654.
Immunogen
Epitope: Phosphorylated Tyr653/Tyr654
KLH-conjugated linear peptide corresponding to human FGFR-1 phosphorylated at Tyr653/Tyr654.
Application
Detect phospho-FGFR-1 (Tyr653/Tyr654) using this Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody validated for use in WB, Cell Function Assay.
Peptide Inhibition Assay Analysis: 0.2 µg/mL from a representative lot peptide blocked in HEK293T cells transfected with FGFR-1.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
Cytokines & Cytokine Receptors
Quality
Evaluated by Western Blot in untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR1.
Western Blot Analysis: 0.2 µg/mL of this antibody detected FGFR-1 in 10 µg of untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR1.
Western Blot Analysis: 0.2 µg/mL of this antibody detected FGFR-1 in 10 µg of untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR1.
Target description
~145 kDa observed.
FGFR-1 has 19 isoforms and eight potential glycosylation sites that increase the expected size to 120 kDa for the immature form and 145 kDa for the mature form of the receptor (Hisaoka, K., et al. (2011). JBC. 286(24):21118-21128).
FGFR-1 has 19 isoforms and eight potential glycosylation sites that increase the expected size to 120 kDa for the immature form and 145 kDa for the mature form of the receptor (Hisaoka, K., et al. (2011). JBC. 286(24):21118-21128).
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR-1
Untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR-1
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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LTBP-2 Has a Single High-Affinity Binding Site for FGF-2 and Blocks FGF-2-Induced Cell Proliferation.
Menz, C; Parsi, MK; Adams, JR; Sideek, MA; Kopecki, Z; Cowin, AJ; Gibson, MA
Testing null
Agata Lampart et al.
Cells, 11(3) (2022-02-16)
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EBioMedicine, 53, 102683-102683 (2020-03-03)
Fibroblast growth factor receptor (FGFR)1 and FGFR4 have been associated with tumorigenesis in a variety of tumour types. As a therapeutic approach, their inhibition has been attempted in different types of malignancies, including lung cancer, and was initially focused on
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