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Key Documents

06-727

Sigma-Aldrich

Anti-FcεRI Antibody, γ subunit

Upstate®, from rabbit

Synonym(s):

High affinity immunoglobulin epsilon receptor subunit gamma, Fc-epsilon RI-gamma, Fc receptor gamma-chain, FcR gamma, IgE Fc receptor subunit gamma

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat, mouse

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
western blot: suitable

isotype

IgG

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... FCER1A(2205)

Related Categories

General description

High affinity immunoglobulin epsilon receptor subunit gamma (UniProt P20411; also known as Fc-epsilon RI-gamma, Fc receptor gamma-chain, FceRI gamma, FcR gamma, IgE Fc receptor subunit gamma) is encoded by the Fcer1g (also known as Fce1g) gene (Gene ID: 25441) in rat species. FceR1 gamma is a single-pass type I membrane protein that associates with a variety of FcR alpha chains to form a functional signaling complex that regulates several aspects of the immune response. The gamma subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface. FceRI gamma is found exclusively on mast cells and basophils. In rodents Fc epsilon RI is a tetrameric complex of non-covalently attached subunits: one IgE-binding alpha subunit, one beta subunit and a dimer of disulfide-linked gamma subunits. When multivalent allergens bind to the receptor-bound IgE, the consequent aggregation of the receptors leads to the release of mediators responsible for allergic symptoms. (Ref.: Blank, U., et al. (1989). Nature 337(6203); 187-189).

Specificity

Other species cross-reactivity not tested.
Recognizes and is specific for FcεRI, γ-subunit, MW 10kDa.

Immunogen

KLH-conjugated linear peptide corresponding to 7 amino acids from the C-terminal region of rat FceRI gamma subunit.

Application

Anti-FceRI, gamma subunit, Cat. No. 06-727, is a rabbit polyclonal antibody that detects FceRI, subunit and has been tested for use in Flow Cytometry and Western Blotting.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected FceRI, gamma subunit in rat basophilic leukemia cells.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Quality

Evaluated by western blot on RIPA lysate of RBL cells.

Western Blot Analysis: 0.5-2 μg/mL of this antibody detected FcεRI, γ-subunit in RIPA lysate of RBL cells.

Target description

30 kDa

Physical form

Format: Purified
Protein A purified
Purified rabbit polylclonal IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol.

Storage and Stability

Stable for 1 year at from date of receipt.

Analysis Note

Control
RBL cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Linda K Myers et al.
Arthritis and rheumatism, 63(9), 2661-2670 (2011-05-19)
To explore the characteristics of the T cell population that responds to an analog peptide (A9) of type II collagen and regulates autoimmunity, using the collagen-induced arthritis (CIA) model. Analog peptide A9 is a 26-amino acid peptide analogous to the
Characterization of the family of dimers associated with Fc receptors (Fc epsilon RI and Fc gamma RIII)?Regional distribution of Na,K-ATPase activity in porcine lens epithelium?PDZ domain interaction controls the endocytic recycling of the cystic fibros
Letourneur, O., et al
Journal of immunology (Baltimore, Md. : 1950), 147, 2652-2656 (1991)
Yuan-Wei Zhang et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(20), E2776-E2783 (2016-05-04)
Serotonin transporter (SERT) is responsible for reuptake and recycling of 5-hydroxytryptamine (5-HT; serotonin) after its exocytotic release during neurotransmission. Mutations in human SERT are associated with psychiatric disorders and autism. Some of these mutations affect the regulation of SERT activity
TGF-beta 1 inhibits mast cell Fc epsilon RI expression.
Gomez, G; Ramirez, CD; Rivera, J; Patel, M; Norozian, F; Wright, HV; Kashyap et al.
Journal of immunology (Baltimore, Md. : 1950) (1950)
Oxysterol represses high-affinity IgE receptor-stimulated mast cell activation in Liver X receptor-dependent and -independent manners.
Nunomura S, Endo K, Makishima M, Ra C
Febs Letters null

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