Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
species reactivity (predicted by homology)
rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... PRKAA1(5562)
mouse ... Prkaa1(105787)
rat ... Prkaa1(65248)
Related Categories
General description
Specificity
Immunogen
Application
Western Blot Analysis: 2 μg/mL of this antibody detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.
Immunohistochemistry (Paraffin) Analysis: 1:250 dilution of this antibody detected AMPK α1 in human gallbladder tissue sections.
Signaling
Insulin/Energy Signaling
Quality
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.
Target description
Linkage
Physical form
Storage and Stability
Analysis Note
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Other Notes
Legal Information
Disclaimer
recommended
wgk_germany
WGK 1
Certificates of Analysis (COA)
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