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07-577

Sigma-Aldrich

Anti-Stargazin/Cacng2 Antibody, CT

Upstate®, from rabbit

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Synonym(s):
Neuronal voltage-gated calcium channel gamma-2 subunit, calcium channel, voltage-dependent, gamma subunit 2, voltage-dependent calcium channel gamma-2 subunit
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

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This Item
MABN863SAB2100330AV34957
vibrant-m

07-577

Anti-Stargazin/Cacng2 Antibody, CT

species reactivity

rat, mouse

species reactivity

human

species reactivity

human

species reactivity

horse, human, guinea pig, rat, mouse, bovine, rabbit, dog

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

UniProt accession no.

Q9Y698

UniProt accession no.

Q13936

UniProt accession no.

Q08289

UniProt accession no.

P54284

technique(s)

immunocytochemistry: suitable, western blot: suitable, immunoprecipitation (IP): suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

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General description

Stargazin belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state.
There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.

Specificity

Predicted to cross-react with mouse based on sequence similarity.
Recognizes Stargazin, MW ~36-39 kDa.

Immunogen

Epitope: C-terminus
KLH-conjugated, synthetic peptide corresponding to amino acids 305-323 (KDSKDSLHANTANRRTTPV) of rat Stargazin.

Application

Immunoprecipitation:
2-5 µg of this antibody has been reported by an independent laboratory to immunoprecipitate Stargazin in rat P2 lysate.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
This Anti-Stargazin/Cacng2 Antibody, CT is validated for use in IC, IP, WB for the detection of Stargazin/Cacng2.

Quality

Evaluated by western blot on RIPA lysates from rat brain microsomal preparation (Catalog # 12-144).

Western Blot Analysis:
0.5-2.0 µg/mL of this antibody detected Stargazin in RIPA lysates from rat brain microsomal preparation (Catalog # 12-144).

Target description

~36-39 kDa

Physical form

Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Nonchannel functions of the calcium channel gamma subunit: insight from research on the stargazer mutant.
Qiao, Xiaoxi and Meng, Hongdi
Journal of Bioenergetics and Biomembranes, 35, 661-670 (2003)
Maureen G Price et al.
The Journal of biological chemistry, 280(20), 19711-19720 (2005-03-12)
Mutations in the Cacng2 gene encoding the neuronal transmembrane protein stargazin result in recessively inherited epilepsy and ataxia in "stargazer" mice. Functional studies suggest a dual role for stargazin, both as a modulatory gamma subunit for voltage-dependent calcium channels and
L Yu et al.
Neuroscience, 178, 13-20 (2011-01-25)
Changes in AMPA receptors have been proposed to underlie changes in synaptic efficacy in hippocampus and other brain structures. Calpain activation has also been discussed as a potential mechanism to produce lasting modifications of synaptic structure and function. Stargazin is
Mohammad I K Hamad et al.
Development (Cambridge, England), 141(8), 1737-1748 (2014-03-29)
The ionotropic α-amino-3-hydroxy-5-methyl-4-isoxazole propionate glutamate receptors (AMPARs) have been implicated in the establishment of dendritic architecture. The transmembrane AMPA receptor regulatory proteins (TARPs) regulate AMPAR function and trafficking into synaptic membranes. In the current study, we employ type I and
New transmembrane AMPA receptor regulatory protein isoform, gamma-7, differentially regulates AMPA receptors.
Kato, AS; Zhou, W; Milstein, AD; Knierman, MD; Siuda, ER; Dotzlaf, JE; Yu, H; Hale et al.
The Journal of Neuroscience null

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