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Chromocult® RAMBACH Agar

ISO 6579, Specialized media for the isolation and differentiation of Salmonella spp. from food and animal feed, water and other materials.

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Synonym(s):
Chromogenic Salmonella Agar, Propylene Glycol Deoxycholate Neutral Red Agar, Salmonella Agar

agency

ISO 6579
suitable for ISO

Quality Level

form

powder

technique(s)

microbiological culture: suitable

pH

7.3±0.2 (25 °C)

application(s)

food and beverages

storage temp.

15-25°C

suitability

selective for enterobacteriaceae: Salmonella spp.

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1.00188

Chromocult® RAMBACH Agar

vibrant-m

1.46719

Rambach Agar

vibrant-m

1.10747

GranuCult® BPLS agar, modified

agency

ISO 6579, suitable for ISO

agency

ISO 6579

agency

-

agency

APHA, ISO 11290

suitability

selective for enterobacteriaceae: Salmonella spp.

suitability

Salmonella spp.

suitability

Salmonella spp. (spp.)

suitability

Listeria spp.

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

application(s)

food and beverages

application(s)

food and beverages
industrial qc
microbiology

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

General description

Chromocult® RAMBACH Agar is specifically designed for the differentiation of Salmonella species. The agar is chromogenic, providing two chromogenic substrates – propylene glycol and X-Gal ((5-bromo-4-chloro-3-indoyl- β-D-galactopyranoside).
Certain Salmonella species act on the propylene glycol producing acid which causes the pH indicator in the media to change color, and pinkish-red or crimson colonies appear. Additionally, other members of the family Enterobacteriaceae which are positive for the enzyme β-D-galactosidase cleave the substrate X-Gal and this results in the production of blue-green colonies.
Salmonella species that are positive for the β-D-galactosidase enzyme and can produce acid from propylene glycol appear as purple-violet colonies, while species that are capable of neither appear as yellow or colorless colonies.

Application

The Chromocult® RAMBACH Agar can be used for: -
  • The detection of Salmonella in foodstuff, water, animal feed
  • The differential diagnosis of Salmonella species in various samples

Analysis Note

  • The dehydrated medium appears as dark-green color.
  • The prepared medium is opaque and light pink in appearance.
  • The pH value at 25 degrees is in the range of 7.1-7.5.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
Rambach is a trademark of Balel S.A.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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