MilliporeSigma

1.01695

SAFC

Benzonase®

suitable for biopharmaceutical production EMPROVE® bio

Synonym(s):
Endonuclease from Serratia marcescens
CAS Number:
Enzyme Commission number:
MDL number:

biological source

Serratia marcescens

Quality Level

recombinant

expressed in E. coli

product line

EMPROVE® Bio

Assay

≥99% (SDS-PAGE)

form

buffered aqueous glycerol solution

concentration

≥250 units/μL

pH

8.0 (25 °C in H2O)

application(s)

pharma/biopharma processes

shipped in

dry ice

storage temp.

−20°C

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This Item
1.01697E101470746
Benzonase® suitable for biopharmaceutical production EMPROVE® bio

SAFC

1.01695

Benzonase®

Benzonase® endonuclease EMPROVE® EXPERT

SAFC

1.01697

Benzonase® endonuclease

Benzonase® Nuclease ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Millipore

E1014

Benzonase® Nuclease

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥99% (SDS-PAGE)

assay

≥99% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

>90% (SDS-PAGE)

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

concentration

≥250 units/μL

concentration

≥250 units/μL

concentration

≥250 units/μL

concentration

25-29 units/μL

pH

8.0 (25 °C in H2O)

pH

8.0 (25 °C in H2O)

pH

-

pH

-

General description

Benzonase® endonuclease acts as an unspecific endonuclease, degrading both DNA and RNA to small 3–5 base pairs (< 6 kDa) fragments with no base preference. It is the ideal tool for nucleic acid removal in virus vector and vaccine manufacturing as demanded by regulators. Additionally, the use of Benzonase® endonuclease increases the yield in virus purification, protects downstream chromatography and filter devices from fouling, and reduces feed stream viscosity.

Benzonase® endonuclease is the smart solution for DNA removal in viral vaccine and viral vector production and has proven its value for over 30 years. Balancing efficiency and regulatory compliance by delivering reliability and high-quality due manufacturing under good manufacturing practices (GMP ICH Q7). Our extensive documentation packages of Emprove® dossiers and the availability of a FDA Drug Master File type II, supports you in filing your vaccine manufacturing process to the authorities.
Our SAFC® portfolio of high-quality products for biopharmaceutical processing withstands strict quality control procedures and is produced according to MQ-500 requirements as defined by the M-Clarity program.

M-Clarity Program

As part of our EMPROVE® Program, our raw materials are offered with EMPROVE® Dossiers which provide comprehensive, up-to-date documentation to help you navigate regulatory challenges, manage risks, and improve your manufacturing processes.

Our comprehensive portfolio of downstream process chemicals not only provides biopharmaceutical manufacturers with high-quality raw materials for production of classical and novel therapies, but also helps them get to market faster and simplify regulatory challenges. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

Used for the removal of nucleic acid from protein samples.

Biochem/physiol Actions

Digests native or heat-denatured DNA and RNA.

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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Customers Also Viewed

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Articles

Removing DNA and RNA Using Benzonase Endonuclease During Viral Production

Whether you are developing cell and gene therapies or viral vaccines, removing contaminating nucleic acids is an important part of viral production. The simplest way to do this is to use the Benzonase® endonuclease.

Virus-like Particle (VLP) Vaccine Bioprocessing and Formulation Steps

This technical article breaks down the steps of upstream and downstream bioprocessing and formulation of virus-like particle vaccines.

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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