1.05448

Wort agar

GranuCult^® plus, suitable for microbiology, for yeasts, for molds

• Synonym(s): Selective media for yeasts and molds, Yeasts and molds medium
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 200
• form: granular
• packaging: pkg of 5 kg; pkg of 500 g
• manufacturer/tradename: GranuCult^® plus
• storage condition: dry media (Store tightly closed)
• technique(s): microbiological culture: suitable
• color: beige
• pH: 5.0 (30 °C, 55 g/L in H₂O, after autoclaving)
• solubility: 55 g/L
• bulk density: 520 kg/m³
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: molds; yeasts

Description

General description

Wort agar is used for the cultivation, isolation, and enumeration or enrichment of yeasts. The addition of certain indicator dyes to the medium allows differentiation between yeast and bacterial colonies. The accompanying bacterial flora is weakly suppressed by a pH value of 5.0 and largely by a pH of 3.5.

Application

Wort agar is suitable for the cultivation, isolation, and enumeration or enrichment of fungi, especially of yeasts.

Features and Benefits

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years•High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (colour): brownish
Solidification behaviour (2 hrs., 45 °C): liquid
pH-value (25 °C): 4.8 - 5.2
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): ≥ 70 %
Recovery on test medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): ≥ 70 %
Recovery on test medium (Saccharomyces cerevisiae ATCC 9080): ≥ 70 %
Recovery on test medium (Rhodotorula mucilaginosa DSM 70403): ≥ 70 %
Growth (Geotrichum candidum DSM 1240): good to very good
Growth (Penicillium commune ATCC 10428): fair to very good
Growth (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): good to very good
Growth (Trichophyton ajelloi ATCC 28454): fair to good
Incubation: 7 days; 28 °C; aerobic (yeasts 3 days).

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3