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1.05568

Supelco

TLC aluminum sheets, Kieselguhr F₂₅₄

pkg of 25 sheets, sheet L × W 20 cm × 20 cm, aluminum support

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Synonym(s):
Kieselguhr Coated TLC Plates
UNSPSC Code:
41115711
NACRES:
NB.21

material

aluminum support
silica gel 60 matrix

Quality Level

feature

binder Organic Polymer
fluorescent indicator

packaging

pkg of 25 sheets

technique(s)

thin layer chromatography (TLC): suitable

layer thickness

200 μm

sheet L × W

20 cm × 20 cm

particle size

10-12 μm

pore size

60 Å medium pore diameter

storage temp.

2-30°C

General description

25 Aluminium sheets 20 x 20 cm
Precoated Kieselguhr or mixed Silica gel/Kieselguhr plates are only of small importance nowadays. Kieselguhr layers are used like cellulose layers for the TLC separation of polar and moderately polar substances. Polyamide, on the other hand , is used for the separation of flavonoids and other phenolic substances.

Linkage

Replaces: 5568-7; 5568

Analysis Note

Layer thickness: 170 - 220 µm
Deviation of layer thickness per plate: ≤ 30 µm
Chromatographic testing:
amino acid test
hRf-values
- β-alanine, short-time test:
25 - 30
- L-alanine, amino acid test: 45 - 55
- DL-Valin, amino acid test: 75 - 85
- L-leucine, amino acid test: 85 - 95
Typical value determined on a sheet
Eluent: n- propanol/ water (80/20 v/v)

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