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GranuCult® VRBD (Violet Red Bile Dextrose) agar

acc. EP, USP, JP and ISO 21528, For the detection and enumeration of Enterobacteriaceae

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Synonym(s):
VRBG Agar. Violet Red Bile Glucose Agar. Crystal-violet neutral-red bile dextrose agar, VRBD-agar (Crystal-violet neutral-red bile dextrose agar) acc. to MOSSEL

agency

APHA
EP
ISO 21528
JP
USP

Quality Level

form

medium granules (dehydrated (DCM))

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

7.3 (37 °C, 40 g/L in H2O, after autoclaving)

solubility

39.5 g/L

bulk density

720 kg/m3

application(s)

food and beverages
microbiology
pharmaceutical

storage temp.

15-25°C

suitability

enterobacteriaceae

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This Item
1.464351.038541.16122
form

medium granules (dehydrated (DCM))

form

-

form

powder

form

medium granules (dehydrated (DCM))

agency

APHA, ISO 21528, USP, EP, JP

agency

ISO 21528

agency

ISO 21872

agency

ISO 16649

suitability

enterobacteriaceae

suitability

enterobacteriaceae

suitability

selective and differential for Vibrio spp.

suitability

Escherichia coli

manufacturer/tradename

GranuCult®

manufacturer/tradename

ReadyTube®

manufacturer/tradename

-

manufacturer/tradename

ChromoCult®

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

General description

Violet Red Bile Dextrose Agar is a granulated selective medium recommended for detection and enumeration of Enterobacteriaceae, especially the bile tolerant gram-negative bacteria from non-sterile products and pharmaceutical preparations. The granules ensure rapid and uniform dissolution in water, prevent clumping of the medium, and inhalation of the airborne powder. Pancreatic digest of gelatin and yeast extract provide nitrogenous compounds and other nutrients essential for bacterial metabolism. This media is selective due to the presence of the inhibitors, bile salts, and crystal violet. Crystal violet inhibits gram-positive organisms. The neutral red indicator helps to detect dextrose fermentation. Dextrose fermenting strains produce red colonies with pink-red halos in the presence of neutral red. Sodium chloride maintains the osmotic equilibrium in the medium.

Application

Violet Red Bile Dextrose Agar is recommended for the detection and enumeration of Enterobacteriaceae from pharmaceutical products.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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Articles

Microbial culture media is available in both powdered and granulated forms. This article compares the characteristics of each culture media format with regards to safety, handling and convenience.

Learn more about our culture media portfolio for microbial examination of non-sterile products, fully compliant with the harmonized Pharmacopoeia.

Protocols

The International Organization for Standardization (ISO) published the revised EN ISO 21528-1 and EN ISO 21528-2 standards in 2017.

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