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Millipore

OF basal medium

for the differentiation and classification of gram-negative intestinal bacteria

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Synonym(s):
OF test medium, Oxidation-fermentation test medium acc. to HUGH and LEIFSON

Quality Level

form

powder (dehydrated (DCM))

technique(s)

microbiological culture: suitable

pH

7.0-7.2 (37 °C, 12 g/L in H2O, after autoclaving)

solubility

12 g/L

bulk density

770 kg/m3

application(s)

clinical
microbiology

storage temp.

15-25°C

suitability

enterobacteriaceae

General description

for the differentiation and classification of gram-negative intestinal bacteria

Analysis Note

Appearance (clearness)clearAppearance (colour)dark green to blue-greenpH-value (25 °C)6.9 - 7.3Growth aerobic (Escherichia coli ATCC 25922 (WDCM 00013))good to very goodGrowth aerobic (Staphylococcus aureus ATCC 25923 (WDCM 00034))good to very goodGrowth aerobic (Kocuria rhizophila ATCC 9341)good to very goodGrowth aerobic (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))good to very goodGrowth aerobic (Alcaligenes faecalis ATCC 19209)good to very goodGrowth aerobic (Pseudomonas alcaligenes ATCC 14909)good to very goodColour change to yellow (Escherichia coli ATCC 25922 (WDCM 00013))+Colour change to yellow (Staphylococcus aureus ATCC 25923 (WDCM 00034))+Colour change to yellow (Kocuria rhizophila ATCC 9341)+Colour change to yellow (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))+Colour change to yellow (Alcaligenes faecalis ATCC 19209)-Colour change to yellow (Pseudomonas alcaligenes ATCC 14909)-Colour change to yellow anaerobic (Escherichia coli ATCC 25922 (WDCM 00013))+Colour change to yellow anaerobic (Staphylococcus aureus ATCC 25923 (WDCM 00034))+Colour change to yellow anaerobic (Kocuria rhizophila ATCC 9341)(-)Colour change to yellow anaerobic (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))-Colour change to yellow anaerobic (Alcaligenes faecalis ATCC 19209)-Colour change to yellow anaerobic (Pseudomonas alcaligenes ATCC 14909)-Incubation: E.coli ATCC 25922 and Pseud.aerug ATCC 27853 24 hrs; 37 °C; aerobic; other strains 48 hrs.; 35 °C; aerobic.
Tested with D(+)glucose.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2


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