1.10824
Granucult® Modified UVM broth (Base)
Listeria selective enrichment, According to USDA-FSIS; For microbiology
Synonym(s):
Listeria Enrichment Medium Base, UVM Broth, University of Vermont Medium, modified
About This Item
Recommended Products
agency
USDA-FSIS
Quality Level
form
medium granules (dehydrated (DCM))
manufacturer/tradename
GranuCult®
technique(s)
microbiological culture: suitable
pH
7.0-7.4 (25 °C, 54.4 g/L in H2O, after autoclaving)
solubility
54.4 g/L
application(s)
food and beverages
microbiology
pathogen testing
storage temp.
15-25°C
suitability
Listeria spp.
General description
Application
Linkage
Analysis Note
Appearance (color): yellowish-brown
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≤ 100
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≥ 10000
Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≥ 10000
Growth (Listeria monocytogenes ATCC 35152 (WDCM 00109)): >10 colonies on ALOA medium, blue green with opaque halo
Growth (Listeria monocytogenes ATCC 13932 (WDCM 00021)): >10 colonies on ALOA medium, blue green with opaque halo
Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032)): <100 colonies on TSA
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): <100 colonies on TSA
Mixed inoculum of Listeria and S.aureus
Additionaly monoculture of S.aureus and B.cereus
Confirmation on ALOA medium resp. Tryptic soy agar
Incubation: 20 - 26 hours at 30 ± 2°C, aerobic
Confirmation on ALOA medium: 44 ± 4 hours at 37 ±1°C
Confirmation on Tryptic soy agar: 24 ± 3 hours at 37 ± 1°C
Footnote
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
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