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1.10824

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Granucult® Modified UVM broth (Base)

Listeria selective enrichment, According to USDA-FSIS; For microbiology

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Synonym(s):
Listeria Enrichment Medium Base, UVM Broth, University of Vermont Medium, modified
UNSPSC Code:
41106213
NACRES:
NA.74

agency

USDA-FSIS

Quality Level

200

form

medium granules (dehydrated (DCM))

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

7.0-7.4 (25 °C, 54.4 g/L in H2O, after autoclaving)

solubility

54.4 g/L

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

15-25°C

suitability

Listeria spp.

Related Categories

General description

Granucult® Modified UVM broth (Base) is suitable for the selective enrichment of Listeria in the two-stage procedure. The combination of various peptones, extracts, salts, and buffer substances enables the good growth of Listeria. The selectivity is due to the antiproliferative substances nalidixic acid and acriflavine hydrochloride. The two-stage enrichment method has demonstrated its value, especially with sample materials (meat and meat products) that are characterized by a high level of accompanying flora.

Application

Granucult® Modified UVM broth (Base) is suitable for the selective enrichment of Listeria in foods and beverages.

Linkage

Replaces: 1.10824.5007

Analysis Note

Appearance (clearness): clear to turbid
Appearance (color): yellowish-brown
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≤ 100
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≥ 10000
Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≥ 10000
Growth (Listeria monocytogenes ATCC 35152 (WDCM 00109)): >10 colonies on ALOA medium, blue green with opaque halo
Growth (Listeria monocytogenes ATCC 13932 (WDCM 00021)): >10 colonies on ALOA medium, blue green with opaque halo
Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032)): <100 colonies on TSA
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): <100 colonies on TSA
Mixed inoculum of Listeria and S.aureus
Additionaly monoculture of S.aureus and B.cereus
Confirmation on ALOA medium resp. Tryptic soy agar
Incubation: 20 - 26 hours at 30 ± 2°C, aerobic
Confirmation on ALOA medium: 44 ± 4 hours at 37 ±1°C
Confirmation on Tryptic soy agar: 24 ± 3 hours at 37 ± 1°C

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

Certificates of Analysis (COA)

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