MilliporeSigma
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1.12084

Sigma-Aldrich

HEMATOGNOST Fe

staining kit for the detection of free ionic iron (Fe³⁺) in cells

Quality Level

IVD

for in vitro diagnostic use

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

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This Item
1.159731.004971.01641
HEMATOGNOST Fe™ staining kit for the detection of free ionic iron (Fe³⁺) in cells

Sigma-Aldrich

1.12084

HEMATOGNOST Fe

AFB-Color modified Staining kit for the detection of acid-fast bacteria (AFB) by hot staining method

Sigma-Aldrich

1.00497

AFB-Color modified

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

application(s)

clinical testing
diagnostic assay manufacturing
hematology

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology, detection

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

General description

HEMATOGNOST Fe - staining kit for the detection of free ionic iron (Fe3+) in cells, is used for human-medical cell diagnosis and serves the purpose of the hematological and histological investigation of sample material of human origin. HEMATOGNOST Fe is a staining kit for performing the so-called Prussian blue (Berlin blue) reaction in hematological and histological specimen material. This staining kit contains all the reagents necessary for the detection of free ionic iron. In the Prussian blue reaction, ionic iron (Fe3+) not bound to the heme structure reacts with potassium hexacyanoferrate(II) in hydrochloric solution. It precipitates as an insoluble complex salt in the blood, bone-marrow, or tissue cells and thus localizes free cellular iron: 4 Fe3+ + 3 K4Fe(CN)6 = Fe4[Fe(CN)6]3 + 12 K+
As a measure to achieve the best possible visual differentiation of the iron deposits in the cytoplasma, this is counterstained with nuclear fast red solution (Nuclear fast red-aluminum sulfate solution 0.1% (Product number 1.00121), resulting in a tender pink color. If further, more detailed information on the cell morphology is required, counterstaining with any of the other familiar “classical” methods such as Giemsa, (Giemsa, Product number 1.09204), May-Grünwald (May-Grünwald eosin methylene blue solution, Product number 1.01424), hemalum (Mayer′s hemalum solution, Product numer 1.09249), etc. can also be employed.
The Prussian blue reaction can also be used to stain specimens already stained by one of these classical procedures for the additional detection of iron. Repeat counterstaining is not necessary in this case.
The staining kit is sufficient for 6 - 8 stainings with up to 16 slides. The staining kit is developed and registered as CE / IVD product for applications in diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Potassium hexacyanoferrate II (Reagent 1): 4.5 - 5.5 %
Hydrochloric acid (Reagent 2): 4.5 - 5.5 %
Density (Reagent 3 d 20 °C/4 °C): 1.040 - 1.060
Suitability for microscopy: passes test

Legal Information

HEMATOGNOST FE is a trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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