MilliporeSigma
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125193

SAFC

Cellvento® 4HEK Liquid

Chemically defined, sterile; sterile-filtered, suitable (for HEK293 cell culture)

All Photos(1)

Quality Level

500

sterility

sterile; sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

suitability

suitable (for HEK293 cell culture)

shipped in

ambient

storage temp.

2-8°C

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1251951.037961.03795
Cellvento® 4HEK Liquid Chemically defined, sterile; sterile-filtered, suitable (for HEK293 cell culture)

SAFC

125193

Cellvento® 4HEK Liquid

Cellvento® 4HEK Powder Chemically defined, powder, suitable (for HEK293 cell culture)

SAFC

125195

Cellvento® 4HEK Powder

Cellvento® 4Feed COMP chemically defined cell culture feed

SAFC

1.03796

Cellvento® 4Feed COMP

Cellvento® 4CHO COMP chemically defined cell culture medium, ≤1.0 IU/mL endotoxin, pH 5.1-5.6(without supplement), pH 6.7-7.3(with supplement)

SAFC

1.03795

Cellvento® 4CHO COMP

sterility

sterile; sterile-filtered

sterility

-

sterility

-

sterility

-

form

liquid

form

dry, powder

form

dry powder

form

dry powder

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

-

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

suitability

suitable (for HEK293 cell culture)

suitability

suitable (for HEK293 cell culture)

suitability

-

suitability

-

shipped in

ambient

shipped in

-

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Cellvento® 4HEK Medium is a chemically defined medium optimized for adeno-associated virus (AAV) production in multiple human embryonic kidney (HEK) 293 lineages. Cellvento® 4HEK Medium has been streamlined and cell culture media components incorporated to increase media stability and reduce protein oxidation. The formulation is animal component free.

Application

Cellvento® 4HEK Medium can be used as amplification and production medium for gene therapy applications. This medium enables low doubling time and high viable cell densities with multiple HEK293 cell lines. Cellvento®4HEK Medium is compatible with commonly used transfection reagents like PEI, supports AAV production and facilitates high titers of full capsids in various HEK293 lineages. This easy-to-use medium can be used for all stages like growth, transfection, and production.

Preparation Note

Liquid medium must be supplemented with 6-10 mM L-Glutamine.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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