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14-444-M

Sigma-Aldrich

PKC θ Protein, active, 10 µg

Active, recombinant full-length human PKC theta with a C-terminal 6His-tag. For use in Kinase Assays.

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eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

recombinant

expressed in Sf21 cells

mol wt

Mw 83 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

UniProt accession no.

Gene Information

human ... PRKCQ(5588)

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14-497-M14-483-M14-484-M
PKC θ Protein, active, 10 µg Active, recombinant full-length human PKC theta with a C-terminal 6His-tag. For use in Kinase Assays.

14-444-M

PKC θ Protein, active, 10 µg

Sigma-Aldrich

14-497-M

PKC η Protein, active, 10 µg

Sigma-Aldrich

14-483-M

PKCγ Protein, active, 10 µg

PKCα Protein, active, 10 µg Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

14-484-M

PKCα Protein, active, 10 µg

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

biological source

human

biological source

human

biological source

human

biological source

human

mol wt

Mw 83 kDa

mol wt

Mw 78.6 kDa

mol wt

Mw 79.5 kDa

mol wt

Mw 78 kDa

UniProt accession no.

Q04759

UniProt accession no.

P24723

UniProt accession no.

P05129

UniProt accession no.

P17252

Gene Information

human ... PRKCQ(5588)

Gene Information

human ... PRKCH(5583)

Gene Information

-

Gene Information

human ... PRKCA(5578)

General description

Product Source: expressed in Sf21 cells
Recombinant full-length human PKC theta with a C-terminal 6His-tag

PKC Lipid activator (Catalog # 20-133) is required for use.

Biochem/physiol Actions

Protein Target: PKCθ
Target Sub-Family: AGC

Quality

routinely evaluated by phosphorylation of Histone H1 substrate in vitro

Physical form

immobilized nickel affinity chromatography

Storage and Stability

6 months at -70 °C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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G Baier et al.
The Journal of biological chemistry, 268(7), 4997-5004 (1993-03-05)
Members of the protein kinase C (PKC) family of serine/threonine kinases play a key role in regulating the differentiation and growth of diverse cell types and, to date, the cloning of seven mammalian PKC genes encoding eight distinct isoforms has
B Bauer et al.
The Journal of biological chemistry, 276(34), 31627-31634 (2001-06-19)
Protein kinase C theta (PKC theta) is known to induce NF-kappa B, an essential transcriptional element in T cell receptor/CD28-mediated interleukin-2 production but also T cell survival. Here we provide evidence that PKC theta is physically and functionally coupled to

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