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14-742-M

Sigma-Aldrich

FGFR2 (N549H) Protein, active, 10 µg

Active, N-terminal, 6His-tagged, recombinant, human FGFR2 (N549H), amino acids 456-770. For use in Kinase Assays.

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eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in baculovirus infected Sf21 cells

specific activity

6250 U/mg

mol wt

Mw 38.1 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

UniProt accession no.

Gene Information

human ... FGFR2(2263)

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recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

mol wt

Mw 38.1 kDa

mol wt

Mw 62 kDa

mol wt

Mw 38 kDa

mol wt

Mw 36.4 kDa

Gene Information

human ... FGFR2(2263)

Gene Information

human ... FGFR1(2260)

Gene Information

human ... FGFR2(2263)

Gene Information

human ... FGFR4(2264)

UniProt accession no.

P21802

UniProt accession no.

P11362

UniProt accession no.

P21802

UniProt accession no.

P22455

General description

N-terminal, 6His-tagged, recombinant, human FGFR2 (N549H), amino acids 456-770.
Product Source: recombinant, human FGFR2 (N549H), amino acids 456-770, expressed by baculovirus in Sf21 insect cells.

Application

Research Category
Metabolism
Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Biochem/physiol Actions

Protein Target: FGFR2
Target Sub-Family: TK

Quality

Routinely evaluated by phosphorylation of 500 μM GGEEEEYFELVKKKK.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of active GFGR2 (N549H).

Unit Definition

Specific Activity: where one unit of FGFR2 (N549H) activity is defined as 1nmol phosphate incorporated into 500μM GGEEEEYFELVKKKK per minute at 30°C with a final ATP concentration of 100µM.

Physical form

10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni2+/NTA-agarose

Storage and Stability

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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