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Chromolith® NH2

L × I.D. 50 mm x 4.6 mm HPLC column

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Chromolith® SpeedROD NH2 50-4.6 HPLC column


PEEK column

Quality Level


suitable for USP L8

product line



endcapped: no


1 ea of

extent of labeling

5% Carbon loading


200 bar pressure
45 °C max. temp.


HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)

column L × I.D.

5 cm × 4.6 mm


monolithic silica

matrix active group

NH2 bonding phase

pore size

13 nm mesoporosity
2 μm macroporosity

operating pH range


separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

storage temp.

no temp limit

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General description

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® NH2 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith® NH2 columns fall under the USP L8 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® NH2 columns are suitable for LC-MS applications.
The column dimension of 50 mm length and 4.6 mm I.D. is the best choice for fast separations on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® NH2 5-4.6 guard cartridges (3 pieces) [1.52030] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Analysis Note

Pressure: ≤ 10 bar
Performance (2-nitroanisol)
Tailing (USP): 0.85 - 1.30 %
Theoretical Plates (N/m) (Cytosine): ≥ 80000
Capacity factor (Cytosine): ≥ 1.1

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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