MilliporeSigma
All Photos(1)

Documents

16-228

Sigma-Aldrich

Anti-Cortactin (p80/85) Antibody, clone 4F11, Alexa Fluor 488

clone 4F11, Upstate®, from mouse

Sign Into View Organizational & Contract Pricing

eCl@ss:
32160702

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4F11, monoclonal

species reactivity

bovine, human, mouse, rat, hamster, avian

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... CTTN(2017)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
05-180-I16-254MAB1501X
clone

4F11, monoclonal

clone

4F11, monoclonal

clone

4D11, monoclonal

clone

C4, monoclonal

conjugate

ALEXA FLUOR 488

conjugate

-

conjugate

ALEXA FLUOR 488

conjugate

ALEXA FLUOR 488

antibody form

purified immunoglobulin

antibody form

-

antibody form

purified immunoglobulin

antibody form

purified antibody

biological source

mouse

biological source

-

biological source

mouse

biological source

mouse

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

-

technique(s)

immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable

technique(s)

immunofluorescence: suitable

Still not finding the right product?  

Give our Product Selector Tool a try.

Specificity

Recognizes Cortactin in rodent and human cells

Immunogen

Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src

Application

Detect Cortactin (p80/85) using this Anti-Cortactin (p80/85) Antibody, clone 4F11, Alexa Fluor 488 validated for use in FC, IF, WB & IC.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Quality

Routinely evaluated by immunoblot.

Target description

Mr 80kDa and in avian cells, Mr 80kDa and 85kDa

Physical form

Protein G Purified

Storage and Stability

1 year at 4°C from date of shipment

Analysis Note

Control
Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor 488-conjugate.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Alexa Fluor is a registered trademark of Molecular Probes, Inc.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

C Dehio et al.
The EMBO journal, 14(11), 2471-2482 (1995-06-01)
Shigella flexneri causes bacillary dysentery in humans by invading epithelial cells of the colon. Cell invasion occurs via bacterium-directed phagocytosis, a process requiring polymerization of actin at the site of bacterial entry. We show that invasion of HeLa cells by
C Guinebault et al.
The Journal of cell biology, 129(3), 831-842 (1995-05-01)
Thrombin-induced accumulation of phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) but not of PtdIns(3,4,5,)P3 is strongly correlated with the relocation to the cytoskeleton of 29% of the p85 alpha regulatory subunit of phosphoinositide 3-kinase (PtdIns 3-kinase) and is accompanied by a significant increase in
Hax-1 is required for Rac1-Cortactin interaction and ovarian carcinoma cell migration.
Gomathinayagam, R; Muralidharan, J; Ha, JH; Varadarajalu, L; Dhanasekaran, DN
Genes & Cancer null
T Katsube et al.
The Journal of biological chemistry, 273(45), 29672-29677 (1998-10-29)
Cortactin is an actin filament-binding protein localizing at cortical regions of cells and a prominent substrate for Src family protein-tyrosine kinases in response to multiple extracellular stimuli. Human cortactin has been identified as a protein product of a putative oncogene
Actions by actin: reciprocal regulation of cortactin activity by tyrosine kinases and F-actin.
Gunst, Susan J
The Biochemical Journal, 380, e7-e8 (2004)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service